DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, HW | ko |
dc.contributor.author | Hsueh, YP | ko |
dc.contributor.author | Yang, FC | ko |
dc.contributor.author | Kim, Eunjoon | ko |
dc.contributor.author | Sheng, M | ko |
dc.date.accessioned | 2007-09-27T15:28:02Z | - |
dc.date.available | 2007-09-27T15:28:02Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2000-05 | - |
dc.identifier.citation | JOURNAL OF NEUROSCIENCE, v.20, no.10, pp.3580 - 3587 | - |
dc.identifier.issn | 0270-6474 | - |
dc.identifier.uri | http://hdl.handle.net/10203/1596 | - |
dc.description.abstract | Members of the postsynaptic density-95 (PSD-95)/SAP90 family of membrane-associated guanylate kinase (MAGUK) proteins function as multimodular scaffolds that organize protein-signaling complexes at neuronal synapses. MAGUK proteins contain PDZ, Src homology 3 (SH3), and guanylate kinase (GK)-like domains, all of which can function as sites for specific protein-protein interactions. We report here a direct protein-protein interaction between the SH3 domain and the GK region in the PSD-95 family of MAGUKs. The SH3 domain of the PSD-95 family appears to have an atypical binding specificity, because the classical SH3 binding (-P-X-X-P-) motif is absent from the GK domain. Although SH3-GK binding can occur in either an intramolecular or intermolecular manner, the intramolecular mode is preferred, possibly because of additional tertiary interactions available when the SH3 and GK domains are adjacent in the same polypeptide. Mutations disrupting the intramolecular SH3-GK interaction do not interfere with PSD-95 association with the K+ channel Kv1.4 or with the GK domain-binding protein GKAP. The same mutations, however, inhibit the clustering of Kv1.4 by PSD-95, suggesting that the intramolecular SH3-GK interaction may modulate the clustering activity of PSD-95. | - |
dc.description.sponsorship | This work was supported by the Brain Science Research Program of Korea Ministry of Science and Technology, Korea Research Foundation Grants 98-019-D00022 and 99-041-D00352, and the Korea Science and Engineering Foundation 1999 grant (E.K.). M.S. is Assistant Investigator of the Howard Hughes Medical Institute. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | SOC NEUROSCIENCE | - |
dc.subject | DENSITY PROTEIN PSD-95 | - |
dc.subject | NITRIC-OXIDE SYNTHASE | - |
dc.subject | TUMOR-SUPPRESSOR GENE | - |
dc.subject | K+ CHANNEL | - |
dc.subject | EXCITATORY SYNAPSES | - |
dc.subject | SHANK FAMILY | - |
dc.subject | MEMBRANE | - |
dc.subject | PDZ | - |
dc.subject | BINDING | - |
dc.subject | COMPLEX | - |
dc.title | An intramolecular interaction between Src homology 3 domain and guanylate kinase-like domain required for channel clustering by postsynaptic density-95/SAP90 | - |
dc.type | Article | - |
dc.identifier.wosid | 000086940000013 | - |
dc.identifier.scopusid | 2-s2.0-0034657662 | - |
dc.type.rims | ART | - |
dc.citation.volume | 20 | - |
dc.citation.issue | 10 | - |
dc.citation.beginningpage | 3580 | - |
dc.citation.endingpage | 3587 | - |
dc.citation.publicationname | JOURNAL OF NEUROSCIENCE | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Kim, Eunjoon | - |
dc.contributor.nonIdAuthor | Shin, HW | - |
dc.contributor.nonIdAuthor | Hsueh, YP | - |
dc.contributor.nonIdAuthor | Yang, FC | - |
dc.contributor.nonIdAuthor | Sheng, M | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | PDZ domain | - |
dc.subject.keywordAuthor | ion channel clustering | - |
dc.subject.keywordAuthor | postsynaptic density | - |
dc.subject.keywordAuthor | Src tyrosine kinase | - |
dc.subject.keywordAuthor | polyproline helix | - |
dc.subject.keywordAuthor | disks large | - |
dc.subject.keywordPlus | DENSITY PROTEIN PSD-95 | - |
dc.subject.keywordPlus | NITRIC-OXIDE SYNTHASE | - |
dc.subject.keywordPlus | TUMOR-SUPPRESSOR GENE | - |
dc.subject.keywordPlus | K+ CHANNEL | - |
dc.subject.keywordPlus | EXCITATORY SYNAPSES | - |
dc.subject.keywordPlus | SHANK FAMILY | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.subject.keywordPlus | PDZ | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | COMPLEX | - |
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