Construction of Escherichia coli - Mannheimia succiniciproducens shuttle vector for genetic engineering

Abstract

Mannheimia succiniciproducens MBEL55E is a capnophilic gram-negative bacteria isolated from Korean Bovine. M. succiniciproducens can produce a large amount of succinic acid as a major fermentation product. The complete genome sequence of M. succiniciproducens was also reported. Based on the genome sequence, genetic engineering is required to reduce the production of other organic acids. pMVSCS1 is a plasmid isolated from Mannheimia varigena, and is known to replicate in M. succiniciproducens. However, cloning genes directly into M. succinciproducens is not reported so far. Therefore we constructed a shuttle vector pME19 which replicates stably in both Escherichia coli and M. succiniciproducens using the replicon of pMVSCS1. This shuttle vector can be used for further genetic engineering of M .succiniciproducens for succinic acid production.