DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Young-Pil | ko |
dc.contributor.author | Cho, Kun | ko |
dc.contributor.author | Lee, Dohoon | ko |
dc.contributor.author | Piao, Yunxian | ko |
dc.contributor.author | Ahn, Yeong Hee | ko |
dc.contributor.author | Yoo, Jong Shin | ko |
dc.contributor.author | Hyun, Taeghwan | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2009-12-07T07:25:02Z | - |
dc.date.available | 2009-12-07T07:25:02Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | RAPID COMMUNICATIONS IN MASS SPECTROMETRY, v.21, no.21, pp.3435 - 3442 | - |
dc.identifier.issn | 0951-4198 | - |
dc.identifier.uri | http://hdl.handle.net/10203/14310 | - |
dc.description.abstract | We demonstrate that magnetic mesocellular carbon foams (Mag-MCF-C) can be effectively used for enrichment and desalting of protein digests or peptides in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The large mesocellular pores and surface area of Mag-MCF-C are likely to mainly contribute to high efficiency in enrichment and desalting of protein digests. The magnetic property of Mag-MCF-C enabled easy and simple enrichment and desalting process comprising adsorption, washing, and separation steps by using an external magnet. Following elution from Mag-MCF-C by using a matrix solution (CHCA in 70% ACN/0.1% TFA), the peptides were subjected to MALDI-MS analysis. As a result, MALDI mass spectra of peptides or tryptic protein digests were distinct even at a peptide concentration as low as 50 pM. The use of Mag-MCF-C resulted in significantly improved sequence coverage for protein identification when compared to other conventional methods. Mag-MCF-C will find applications in mass spectrometric analysis of low abundance peptides or protein digests with high sensitivity. Copyright (C) 2007 John Wiley & Sons, Ltd. | - |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | Wiley-Blackwell | - |
dc.subject | PERFORMANCE LIQUID-CHROMATOGRAPHY | - |
dc.subject | PEPTIDE RETENTION | - |
dc.subject | MALDI-MS | - |
dc.subject | SAMPLE | - |
dc.subject | FABRICATION | - |
dc.subject | PREDICTION | - |
dc.subject | MIXTURES | - |
dc.subject | COLUMNS | - |
dc.title | Efficient enrichment and desalting of protein digests using magnetic mesocellular carbon foams in matrix-assisted laser desorption/ionization mass spectrometry | - |
dc.type | Article | - |
dc.identifier.wosid | 000250608300005 | - |
dc.identifier.scopusid | 2-s2.0-35648991477 | - |
dc.type.rims | ART | - |
dc.citation.volume | 21 | - |
dc.citation.issue | 21 | - |
dc.citation.beginningpage | 3435 | - |
dc.citation.endingpage | 3442 | - |
dc.citation.publicationname | RAPID COMMUNICATIONS IN MASS SPECTROMETRY | - |
dc.identifier.doi | 10.1002/rcm.3231 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.nonIdAuthor | Kim, Young-Pil | - |
dc.contributor.nonIdAuthor | Cho, Kun | - |
dc.contributor.nonIdAuthor | Lee, Dohoon | - |
dc.contributor.nonIdAuthor | Piao, Yunxian | - |
dc.contributor.nonIdAuthor | Ahn, Yeong Hee | - |
dc.contributor.nonIdAuthor | Yoo, Jong Shin | - |
dc.contributor.nonIdAuthor | Hyun, Taeghwan | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | PERFORMANCE LIQUID-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | PEPTIDE RETENTION | - |
dc.subject.keywordPlus | MALDI-MS | - |
dc.subject.keywordPlus | SAMPLE | - |
dc.subject.keywordPlus | FABRICATION | - |
dc.subject.keywordPlus | PREDICTION | - |
dc.subject.keywordPlus | MIXTURES | - |
dc.subject.keywordPlus | COLUMNS | - |
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