DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, Young-Hee | ko |
dc.contributor.author | Kim, Young-Pil | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2009-12-03T07:46:36Z | - |
dc.date.available | 2009-12-03T07:46:36Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007-03 | - |
dc.identifier.citation | BIOCHIP JOURNAL, v.1, no.1, pp.28 - 34 | - |
dc.identifier.issn | 1976-0280 | - |
dc.identifier.uri | http://hdl.handle.net/10203/14109 | - |
dc.description.abstract | The analysis of protein interactions and modifications in a microarray format has become the focus of great interest in researchers in drug discovery, diagnostics, and cell biology, due to the advent of rapid and high-throughput biological assays requiring only a small amount of sample. Herein, we describe a chip-based analysis of sumoylation, the post-translational modification (PTM) process involving the covalent attachment of the small ubiquitin-like modifier (SUMO) family protein to a target protein in a mammalian cell. The protein was expressed using an in vitro translation system and immobilized directly onto a glass slide using epitope tags fused to the protein. In this paper, the microarray-based analysis technique was shown to be a useful strategy for the identification of SUMO target proteins from preexisting protein pools and proteome arrays, in a high-throughput manner. | - |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | Springer | - |
dc.subject | GTPASE-ACTIVATING PROTEIN | - |
dc.subject | COMPLEX | - |
dc.subject | MICROARRAYS | - |
dc.subject | TECHNOLOGY | - |
dc.subject | RANGAP1 | - |
dc.subject | UBC9 | - |
dc.title | SUMO (small ubiquitin-like modifier) chip for analysis of SUMO-conjugation to a target protein | - |
dc.type | Article | - |
dc.identifier.wosid | 000258632200003 | - |
dc.type.rims | ART | - |
dc.citation.volume | 1 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 28 | - |
dc.citation.endingpage | 34 | - |
dc.citation.publicationname | BIOCHIP JOURNAL | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.nonIdAuthor | Oh, Young-Hee | - |
dc.contributor.nonIdAuthor | Kim, Young-Pil | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | PTM (post translational modification) | - |
dc.subject.keywordAuthor | SUMO (small ubiquitin-like modifier) | - |
dc.subject.keywordAuthor | protein microarray | - |
dc.subject.keywordAuthor | cell-free expression system | - |
dc.subject.keywordPlus | GTPASE-ACTIVATING PROTEIN | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | MICROARRAYS | - |
dc.subject.keywordPlus | TECHNOLOGY | - |
dc.subject.keywordPlus | RANGAP1 | - |
dc.subject.keywordPlus | UBC9 | - |
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