DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yang, Jae Seung | ko |
dc.contributor.author | Kim, Hye Jin | ko |
dc.contributor.author | Yun, Cheol-Heui | ko |
dc.contributor.author | Kang, Seok-Seong | ko |
dc.contributor.author | Im, Jintaek | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.contributor.author | Han, Seung Hyun | ko |
dc.date.accessioned | 2009-12-03T07:17:51Z | - |
dc.date.available | 2009-12-03T07:17:51Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007-11 | - |
dc.identifier.citation | JOURNAL OF MICROBIOLOGICAL METHODS, v.71, no.2, pp.141 - 146 | - |
dc.identifier.issn | 0167-7012 | - |
dc.identifier.uri | http://hdl.handle.net/10203/14104 | - |
dc.description.abstract | Vibriocidal antibody assay has been a surrogate standard assay in the evaluation of cholera vaccine efficacy because it has a good correlation with protection. Although the optical density-based vibriocidal assay in a 96-well microtiter-plate format is widely used in clinical trials, it has limitations as vibriocidal titers are altered by incubation time and samples with the same end-point titers could have potentially different vibriocidal kinetics. In the present study, we developed an improved agar-plate assay coupled with an automated colony counting system. Through testing 30 pairs of human sera from vaccinces administered with a cholera vaccine or placebo, these two assays showed good correlations for the vibriocidal titers and fold increases in titers between pre- and post-vaccinated sera as determined by the Pearson correlation coefficient and the Regression coefficient. Notably, the newly-developed semi-automated assay demonstrated that serum samples with the same end-point titers turned out to have distinct vibriocidal kinetics that were not distinguishable by the microtiter-plate assay. The semi-automated assay responded, p specifically to vibrio cholerae but not to irrelevant bacteria such as Salmonella typhi and Escherichia coli. These results demonstrate that the semi-automated assay provides better sensitivity, accuracy, and stability of the assay results with minimized efforts than conventional microtiterplate assay and could provide a useful tool as an in vitro surrogate assay for the evaluation of cholera vaccine efficacy. (c) 2007 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | Elsevier Science Bv | - |
dc.subject | WHOLE-CELL | - |
dc.subject | ANTIBODIES | - |
dc.subject | TRIAL | - |
dc.title | A semi-automated vibriocidal assay for improved measurement of cholera vaccine-induced immune responses | - |
dc.type | Article | - |
dc.identifier.wosid | 000250972300007 | - |
dc.identifier.scopusid | 2-s2.0-35048857794 | - |
dc.type.rims | ART | - |
dc.citation.volume | 71 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 141 | - |
dc.citation.endingpage | 146 | - |
dc.citation.publicationname | JOURNAL OF MICROBIOLOGICAL METHODS | - |
dc.identifier.doi | 10.1016/j.mimet.2007.08.009 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.nonIdAuthor | Yang, Jae Seung | - |
dc.contributor.nonIdAuthor | Kim, Hye Jin | - |
dc.contributor.nonIdAuthor | Yun, Cheol-Heui | - |
dc.contributor.nonIdAuthor | Kang, Seok-Seong | - |
dc.contributor.nonIdAuthor | Im, Jintaek | - |
dc.contributor.nonIdAuthor | Han, Seung Hyun | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | vibrio cholerae | - |
dc.subject.keywordAuthor | improved vibriocidal assay | - |
dc.subject.keywordAuthor | cholera vaccine | - |
dc.subject.keywordPlus | WHOLE-CELL | - |
dc.subject.keywordPlus | ANTIBODIES | - |
dc.subject.keywordPlus | TRIAL | - |
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