The effects of surface roughness on a turbulent boundary layer (TBL) were investigated using particle image velocimetry (PIV). The roughness elements used were periodically arranged two-dimensional spanwise rods, and the roughness height was . Introduction of the roughness elements increased the wake strength and the turbulent stress not only in the roughness sublayer but also in the outer layer. This indicates the existence of interaction between inner and outer layers for 2D rod-roughened wall. Roughness effects on a turbulence structure near the wall were obtained by PIV measurements. Iso-contours of mean velocities and Reynolds stresses in the roughness sublayer showed a very good agreement with previous DNS results.