DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chung, Hyun Jung | ko |
dc.contributor.author | Kim, In Kyoung | ko |
dc.contributor.author | Kim, Taek Gyoung | ko |
dc.contributor.author | Park, Tae Gwan | ko |
dc.date.accessioned | 2009-11-23T07:04:51Z | - |
dc.date.available | 2009-11-23T07:04:51Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2008-05 | - |
dc.identifier.citation | TISSUE ENGINEERING, v.14, no.5, pp.607 - 615 | - |
dc.identifier.issn | 2152-4947 | - |
dc.identifier.uri | http://hdl.handle.net/10203/13131 | - |
dc.description.abstract | Injectable cell therapy would provide a patient-friendly procedure for treatment of degenerated or wounded tissue. Biodegradable injectable porous microspheres were fabricated to use as dual-purpose microcarriers for cell culture and injectable scaffold for tissue regeneration. Gas foaming in a water-in-oil-in-water double emulsion was performed for fabricating the well-interconnected porous microcarriers using poly(lactic-co-glycolic acid) (PLGA). The gas foaming conditions were finely tuned to control the structural and morphological characteristics. Porous microcarriers with a mean size of approximately 175 mu m and an average pore diameter of approximately 29 mu m were produced for cell cultivation and injectable delivery. To promote cell seeding, amine-functionalized porous microcarriers were prepared by blending amine-functionalized PLGA with unreacted PLGA. To assess the porous microcarriers for chondrocyte cultivation, bovine articular chondrocytes were seeded and cultured in vitro in spinner flasks for 4 weeks. Visualization and biochemical analyses of the microcarrier-cell constructs were performed to demonstrate cell proliferation and phenotypic expression. Quantification of deoxyribonucleic acid, glycosaminoglycan, and collagen content showed that much greater cell proliferation and expression of cartilage-specific phenotype were observed for cultures in the following order: amine-functionalized porous microcarriers, porous microcarriers, nonporous microcarriers, and monolayer culture. | - |
dc.description.sponsorship | Ministry of Commerce, Industry and Energy (10011366) and from the Ministry of Science and Technology, National Research Laboratory Program, Republic of Korea. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | MARY ANN LIEBERT INC | - |
dc.title | Highly open porous biodegradable microcarriers: In vitro cultivation of chondrocytes for injectable delivery | - |
dc.type | Article | - |
dc.identifier.wosid | 000256239800004 | - |
dc.identifier.scopusid | 2-s2.0-43649096860 | - |
dc.type.rims | ART | - |
dc.citation.volume | 14 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 607 | - |
dc.citation.endingpage | 615 | - |
dc.citation.publicationname | TISSUE ENGINEERING | - |
dc.identifier.doi | 10.1089/tea.2007.0263 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Chung, Hyun Jung | - |
dc.contributor.localauthor | Park, Tae Gwan | - |
dc.contributor.nonIdAuthor | Kim, In Kyoung | - |
dc.contributor.nonIdAuthor | Kim, Taek Gyoung | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | CELL ATTACHMENT | - |
dc.subject.keywordPlus | POLYMERIC MICROSPHERES | - |
dc.subject.keywordPlus | ROTATING BIOREACTOR | - |
dc.subject.keywordPlus | SCAFFOLDS | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | EXPANSION | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | ACID) | - |
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