DC Field | Value | Language |
---|---|---|
dc.contributor.author | Mok, Hyejung | ko |
dc.contributor.author | Park, Ji Won | ko |
dc.contributor.author | Park, Tae Gwan | ko |
dc.date.accessioned | 2009-11-20T06:31:43Z | - |
dc.date.available | 2009-11-20T06:31:43Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007-09 | - |
dc.identifier.citation | BIOCONJUGATE CHEMISTRY, v.18, no.5, pp.1483 - 1489 | - |
dc.identifier.issn | 1043-1802 | - |
dc.identifier.uri | http://hdl.handle.net/10203/13006 | - |
dc.description.abstract | Green fluorescent protein (GFP) antisense oligodeoxynucleotide (ODN) was covalently conjugated to hyaluronic acid (HA) via a reducible disulfide linkage, and the HA-ODN conjugate was complexed with protamine to increase the extent of cellular uptake and enhance the gene inhibition efficiency of GFP expression. The HA-ODN conjugate formed more stable polyelectrolyte complexes with prolamine as compared to naked ODN, probably because of its increased charge density. The higher cellular uptake of protamine/HA-ODN complexes than that of protamine/naked ODN complexes was attributed to the formation of more compact nanosized complexes (similar to 200 nm in diameter) in aqueous solution. Protamine/HA-ODN complexes also showed a comparable level of GFP gene inhibition to that of cytotoxic polyethylenimine (PEI)/ODN complexes. Since both HA and prolamine are naturally occurring biocompatible materials, the current formulation based on a cleavable conjugation strategy of ODN to HA could be potentially applied as safe and effective nonviral carriers for ODN and siRNA nucleic acid therapeutics. | - |
dc.description.sponsorship | National Research Laboratory project from the Ministry of Science and Technology and the National Cancer Center, Republic of Korea. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | Amer Chemical Soc | - |
dc.subject | MOLECULAR-WEIGHT PROTAMINE | - |
dc.subject | TRANSFECTION EFFICIENCY | - |
dc.subject | FUSOGENIC PEPTIDE | - |
dc.subject | NUCLEIC-ACIDS | - |
dc.subject | DNA DELIVERY | - |
dc.subject | IN-VIVO | - |
dc.subject | MICELLES | - |
dc.subject | COPOLYMERS | - |
dc.subject | CELLS | - |
dc.subject | SIRNA | - |
dc.title | Antisense oligodeoxylutudeotide-conjugated hyaluronic Acid/Protamine nanocomplexes for intracellular gene inhibition | - |
dc.type | Article | - |
dc.identifier.wosid | 000249656100017 | - |
dc.identifier.scopusid | 2-s2.0-34648817050 | - |
dc.type.rims | ART | - |
dc.citation.volume | 18 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 1483 | - |
dc.citation.endingpage | 1489 | - |
dc.citation.publicationname | BIOCONJUGATE CHEMISTRY | - |
dc.identifier.doi | 10.1021/bc070111o | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Park, Tae Gwan | - |
dc.contributor.nonIdAuthor | Mok, Hyejung | - |
dc.contributor.nonIdAuthor | Park, Ji Won | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | MOLECULAR-WEIGHT PROTAMINE | - |
dc.subject.keywordPlus | TRANSFECTION EFFICIENCY | - |
dc.subject.keywordPlus | FUSOGENIC PEPTIDE | - |
dc.subject.keywordPlus | NUCLEIC-ACIDS | - |
dc.subject.keywordPlus | DNA DELIVERY | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | MICELLES | - |
dc.subject.keywordPlus | COPOLYMERS | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | SIRNA | - |
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