DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Bong Soo | ko |
dc.contributor.author | Park, Sangjin | ko |
dc.contributor.author | Lee, Kyung-Bok | ko |
dc.contributor.author | Jon, Sangyong | ko |
dc.contributor.author | Choi, Insung | ko |
dc.date.accessioned | 2009-11-19T08:29:39Z | - |
dc.date.available | 2009-11-19T08:29:39Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007-12 | - |
dc.identifier.citation | BIOINTERPHASES, v.2, no.4, pp.136 - 142 | - |
dc.identifier.issn | 1559-4106 | - |
dc.identifier.uri | http://hdl.handle.net/10203/12928 | - |
dc.description.abstract | The authors report a facile method for the selective immobilization of biomolecules onto a gold surface that was preactivated by a polymeric adlayer. The polymeric adlayer was designed to perform triple functions: high resistance to nonspecific protein adsorption, efficient surface anchoring, and subsequent covalent attachment of biomolecules. For this purpose, a random copolymer, poly (PEGMA- r- NAS), was synthesized by radical polymerization of poly (ethylene glycol) methyl ether methacrylate (PEGMA) and N- acryloxysuccinimide (NAS). In the first step, the polymeric adlayer was formed onto amine-terminated self-assembled monolayers (SAMs) on gold through covalent bond formation between reactive N- hydroxysuccinimide (NHS) ester of the copolymer and the amine of the SAMs. In the second step, amine-bearing biotin as a model biomolecule was covalently attached onto the polymeric adlayer that still contained unreacted NHS esters. The degrees of the binding sensitivity for a target protein and the nonspecific binding for four model proteins on the biotinylated polymeric adlayer were examined by surface plasmon resonance spectroscopy. Finally, the specific immobilization of rhodamin (TRITC)- conjugated streptavidin on the biotinylated polymeric adlayer was achieved by a simple microcontact printing technique, resulting in well-defined patterns of the protein.(c) 2007 American Vacuum Society. | - |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | AVS, EDITORIAL OFFICE | - |
dc.subject | SELF-ASSEMBLED MONOLAYERS | - |
dc.subject | SURFACE-INITIATED POLYMERIZATIONS | - |
dc.subject | TRANSFER RADICAL POLYMERIZATION | - |
dc.subject | C LINKED MONOLAYERS | - |
dc.subject | CARBOXYLIC-ACID | - |
dc.subject | PROTEIN | - |
dc.subject | GOLD | - |
dc.subject | DNA | - |
dc.subject | MICROARRAYS | - |
dc.subject | GLYCOL) | - |
dc.title | Selective immobilization of biomolecules onto an activated polymeric adlayer | - |
dc.type | Article | - |
dc.identifier.wosid | 000256467100006 | - |
dc.type.rims | ART | - |
dc.citation.volume | 2 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 136 | - |
dc.citation.endingpage | 142 | - |
dc.citation.publicationname | BIOINTERPHASES | - |
dc.identifier.doi | 10.1116/1.2801974 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Jon, Sangyong | - |
dc.contributor.localauthor | Choi, Insung | - |
dc.contributor.nonIdAuthor | Park, Sangjin | - |
dc.contributor.nonIdAuthor | Lee, Kyung-Bok | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | SELF-ASSEMBLED MONOLAYERS | - |
dc.subject.keywordPlus | SURFACE-INITIATED POLYMERIZATIONS | - |
dc.subject.keywordPlus | TRANSFER RADICAL POLYMERIZATION | - |
dc.subject.keywordPlus | C LINKED MONOLAYERS | - |
dc.subject.keywordPlus | CARBOXYLIC-ACID | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | GOLD | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | MICROARRAYS | - |
dc.subject.keywordPlus | GLYCOL) | - |
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