DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Soo Hyeon | ko |
dc.contributor.author | Kim, Sun Hwa | ko |
dc.contributor.author | Park, Tae Gwan | ko |
dc.date.accessioned | 2009-11-10T08:28:06Z | - |
dc.date.available | 2009-11-10T08:28:06Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007-06 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.357, no.2, pp.511 - 516 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/10203/12374 | - |
dc.description.abstract | To develop a small interfering RNA (siRNA) delivery system with low cytotoxicity and high transfection efficiency, siRNA was conjugated to poly(ethylene glycol) via a disulfide linkage (siRNA-PEG) to prepare polyelectrolyte complex micelles (PECMs) by condensing with a cationic fusogenic peptide (KALA). The siRNA-PEG conjugate exhibited enhanced resistance to degradation from nucleases. Anionic siRNA-PEG conjugate and cationic KALA, when mixed in an aqueous phase, spontaneously formed nano-sized PECMs (< 200 nm) that have an inner core of charge neutralized siRNA/KALA complex surrounded by a PEG corona. Vascular endothelial growth factor (VEGF) siRNA was used to demonstrate VEGF sequence-specific gene inhibition in prostate carcinoma cells (PC-3 cells). The extent of gene silencing was gradually increased with increasing nitrogen to phosphate (N/P) ratio and the concentration of siRNA-PEG/KALA PECMs. These results suggest that the formulation of siRNA-PEG/KALA PECMs could be widely applied for intracellular delivery of various therapeutic siRNAs. (c) 2007 Elsevier Inc. All rights reserved. | - |
dc.description.sponsorship | Ministry of Science and Technology (National Research Laboratory Project) and the National Cancer Center, South Korea. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | ANTISENSE OLIGONUCLEOTIDE | - |
dc.subject | GLYCOL) CONJUGATE | - |
dc.subject | GENE DELIVERY | - |
dc.subject | IN-VIVO | - |
dc.subject | GLUTATHIONE | - |
dc.subject | OLIGODEOXYNUCLEOTIDE | - |
dc.subject | THERAPEUTICS | - |
dc.subject | RNAS | - |
dc.title | Intracellular siRNA delivery system using polyelectrolyte complex micelles prepared from VEGF siRNA-PEG conjugate and cationic fusogenic peptide | - |
dc.type | Article | - |
dc.identifier.wosid | 000246253700030 | - |
dc.identifier.scopusid | 2-s2.0-34247153120 | - |
dc.type.rims | ART | - |
dc.citation.volume | 357 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 511 | - |
dc.citation.endingpage | 516 | - |
dc.citation.publicationname | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.identifier.doi | 10.1016/j.bbrc.2007.03.185 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Park, Tae Gwan | - |
dc.contributor.nonIdAuthor | Lee, Soo Hyeon | - |
dc.contributor.nonIdAuthor | Kim, Sun Hwa | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | siRNA delivery | - |
dc.subject.keywordAuthor | PEG | - |
dc.subject.keywordAuthor | fusogenic peptide | - |
dc.subject.keywordAuthor | vascular endothelial growth factor | - |
dc.subject.keywordAuthor | polyelectrolyte complex micelles | - |
dc.subject.keywordPlus | ANTISENSE OLIGONUCLEOTIDE | - |
dc.subject.keywordPlus | GLYCOL) CONJUGATE | - |
dc.subject.keywordPlus | GENE DELIVERY | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | GLUTATHIONE | - |
dc.subject.keywordPlus | OLIGODEOXYNUCLEOTIDE | - |
dc.subject.keywordPlus | THERAPEUTICS | - |
dc.subject.keywordPlus | RNAS | - |
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