DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Sun-Hwa | ko |
dc.contributor.author | Jeong, Ji-Hoon | ko |
dc.contributor.author | Lee, Soo-Hyeon | ko |
dc.contributor.author | Kim, Sung-Wan | ko |
dc.contributor.author | Park, Tae-Gwan | ko |
dc.date.accessioned | 2009-11-10T05:27:17Z | - |
dc.date.available | 2009-11-10T05:27:17Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | JOURNAL OF CONTROLLED RELEASE, v.116, no.2, pp.123 - 129 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.uri | http://hdl.handle.net/10203/12345 | - |
dc.description.abstract | fA novel siRNA delivery system based on polyelectrolyte complex (PEC) micelles was introduced in this study. Vascular endothelial growth factor (VEGF) siRNA was conjugated to poly(ethylene glycol) (PEG) via a disulfide linkage (siRNA-PEG). The siRNA-PEG conjugate could form PEC micelles by interacting with cationic polyethylenimine (PEI) as a core forming agent. The VEGF siRNA-PEG/PEI PEC micelles showed greater stability than naked VEGF siRNA against enzymatic degradation. Under a reductive condition similar to cytosolic environment, an intact form of siRNA was released from the siRNA-PEG conjugate by cleavage of the disulfide linkage. The VEGF siRNA-PEG/PEI PEC micelles effectively silenced VEGF gene expression in prostate carcinoma cells (PC-3) up to 96.5% under an optimized formulation condition. They also showed a far superior VEGF gene silencing effect than VEGF siRNA/PEI complexes even in the presence of serum. This study suggests that the siRNA delivery system using VEGF siRNA-PEG/PEI PEC micelles could be potentially applied to RNAi-based anti-angiogenic treatment of cancer in vivo. (c) 2006 Elsevier B.V. All rights reserved. | - |
dc.description.sponsorship | the Ministry of Science and Technology (M10414030002-05N1403-00210), Korea and from the National Institute of Health (CA107070), USA. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | Elsevier Science Bv | - |
dc.subject | ENDOTHELIAL GROWTH-FACTOR | - |
dc.subject | POLYELECTROLYTE COMPLEX MICELLES | - |
dc.subject | SMALL INTERFERING RNA | - |
dc.subject | IN-VIVO | - |
dc.subject | CHEMICAL-MODIFICATIONS | - |
dc.subject | POLYETHYLENE-GLYCOL | - |
dc.subject | MAMMALIAN-CELLS | - |
dc.subject | CANCER | - |
dc.subject | DELIVERY | - |
dc.subject | THERAPEUTICS | - |
dc.title | PEG conjugated VEGF siRNA for anti-angiogenic gene therapy | - |
dc.type | Article | - |
dc.identifier.wosid | 000245332000004 | - |
dc.identifier.scopusid | 2-s2.0-33845216721 | - |
dc.type.rims | ART | - |
dc.citation.volume | 116 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 123 | - |
dc.citation.endingpage | 129 | - |
dc.citation.publicationname | JOURNAL OF CONTROLLED RELEASE | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Park, Tae-Gwan | - |
dc.contributor.nonIdAuthor | Kim, Sun-Hwa | - |
dc.contributor.nonIdAuthor | Jeong, Ji-Hoon | - |
dc.contributor.nonIdAuthor | Kim, Sung-Wan | - |
dc.type.journalArticle | Article; Proceedings Paper | - |
dc.subject.keywordAuthor | gene delivery | - |
dc.subject.keywordAuthor | vascular endothelial growth factor | - |
dc.subject.keywordAuthor | siRNA-PEG conjugate | - |
dc.subject.keywordAuthor | polyelectrolyte complex micelles | - |
dc.subject.keywordPlus | ENDOTHELIAL GROWTH-FACTOR | - |
dc.subject.keywordPlus | POLYELECTROLYTE COMPLEX MICELLES | - |
dc.subject.keywordPlus | SMALL INTERFERING RNA | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | CHEMICAL-MODIFICATIONS | - |
dc.subject.keywordPlus | POLYETHYLENE-GLYCOL | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | THERAPEUTICS | - |
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