DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Ho Min | ko |
dc.contributor.author | Park, Beom Seok | ko |
dc.contributor.author | Kim, Jung-In | ko |
dc.contributor.author | Kim, Sung Eun | ko |
dc.contributor.author | Lee, Judong | ko |
dc.contributor.author | Oh, Se Cheol | ko |
dc.contributor.author | Enkhbayar, Purevjav | ko |
dc.contributor.author | Matsushima, Norio | ko |
dc.contributor.author | Lee, Hayyoung | ko |
dc.contributor.author | Yoo, Ook-Joon | ko |
dc.contributor.author | Lee, Jie-Oh | ko |
dc.date.accessioned | 2009-09-11T06:08:49Z | - |
dc.date.available | 2009-09-11T06:08:49Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007-09 | - |
dc.identifier.citation | CELL, v.130, no.5, pp.906 - 917 | - |
dc.identifier.issn | 0092-8674 | - |
dc.identifier.uri | http://hdl.handle.net/10203/11149 | - |
dc.description.abstract | TLR4 and MD-2 form a heterodimer that recognizes LPS (lipopolysaccharide) from Gram-negative bacteria. Eritoran is an analog of LPS that antagonizes its activity by binding to the TLR4-MD- 2 complex. We determined the structure of the full-length ectodomain of the mouse TLR4 and MD-2 complex. We also produced a series of hybrids of human TLR4 and hagfish VLR and determined their structures with and without bound MD-2 and Eritoran. TLR4 is an atypical member of the LRR family and is composed of N-terminal, central, and C-terminal domains. The beta sheet of the central domain shows unusually small radii and large twist angles. MD-2 binds to the concave surface of the N-terminal and central domains. The interaction with Eritoran is mediated by a hydrophobic internal pocket in MD-2. Based on structural analysis and mutagenesis experiments on MD-2 and TLR4, we propose a model of TLR4-MD-2 dimerization induced by LPS. | - |
dc.description.sponsorship | Wethank the staff of the Pohang Accelerator Laboratory, Spring-8, and ESRF for help with data collection.Wethank Dr. Julian Gross for critical reading of the manuscript. This work was supported by the Molecular and Cellular BioDiscovery Program and the Functional Proteomics Program (FPR05B2-140) from the Ministry of Science of Korea. Received: June 22, 2007 Revised: July 23, 2007 Accepted: August 2, 2007 Published: September 6, 2007 | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | CELL PRESS | - |
dc.title | Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist eritoran | - |
dc.type | Article | - |
dc.identifier.wosid | 000249581500021 | - |
dc.identifier.scopusid | 2-s2.0-34548222514 | - |
dc.type.rims | ART | - |
dc.citation.volume | 130 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 906 | - |
dc.citation.endingpage | 917 | - |
dc.citation.publicationname | CELL | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Kim, Ho Min | - |
dc.contributor.localauthor | Yoo, Ook-Joon | - |
dc.contributor.localauthor | Lee, Jie-Oh | - |
dc.contributor.nonIdAuthor | Park, Beom Seok | - |
dc.contributor.nonIdAuthor | Kim, Jung-In | - |
dc.contributor.nonIdAuthor | Kim, Sung Eun | - |
dc.contributor.nonIdAuthor | Lee, Judong | - |
dc.contributor.nonIdAuthor | Oh, Se Cheol | - |
dc.contributor.nonIdAuthor | Enkhbayar, Purevjav | - |
dc.contributor.nonIdAuthor | Matsushima, Norio | - |
dc.contributor.nonIdAuthor | Lee, Hayyoung | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | MOLIMMUNO | - |
dc.subject.keywordAuthor | PROTEINS | - |
dc.subject.keywordPlus | TOLL-LIKE RECEPTORS | - |
dc.subject.keywordPlus | CONFERS LIPOPOLYSACCHARIDE RESPONSIVENESS | - |
dc.subject.keywordPlus | LEUCINE-RICH REPEAT | - |
dc.subject.keywordPlus | BACTERIAL LIPOPOLYSACCHARIDE | - |
dc.subject.keywordPlus | MONOMERIC ENDOTOXIN | - |
dc.subject.keywordPlus | PROTEIN COMPLEXES | - |
dc.subject.keywordPlus | CELL ACTIVATION | - |
dc.subject.keywordPlus | MD-2 BINDS | - |
dc.subject.keywordPlus | LIPID IVA | - |
dc.subject.keywordPlus | TLR4 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.