DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ryu, Kyoung-Seok | ko |
dc.contributor.author | Lee, Jie-Oh | ko |
dc.contributor.author | Kwon, Taek Hun | ko |
dc.contributor.author | Choi, Han-Ho | ko |
dc.contributor.author | Park, Hong-Seog | ko |
dc.contributor.author | Hwang, Soo Kyung | ko |
dc.contributor.author | Lee, Zee-Won | ko |
dc.contributor.author | Lee, Kyung-Bok | ko |
dc.contributor.author | Han, Young Hyun | ko |
dc.contributor.author | Choi, Yun-Seok | ko |
dc.contributor.author | Jeon, Young Ho | ko |
dc.contributor.author | Cheong, Chaejoon | ko |
dc.contributor.author | Kim, Soohyun | ko |
dc.date.accessioned | 2009-09-09T02:00:23Z | - |
dc.date.available | 2009-09-09T02:00:23Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2009-07 | - |
dc.identifier.citation | BIOCHEMICAL JOURNAL, v.421, pp.87 - 96 | - |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.uri | http://hdl.handle.net/10203/11091 | - |
dc.description.abstract | Although N-glycosylation has been known to increase the stability of glycoproteins, it is difficult to assess the structural importance of glycans in the stabilization of glycoproteins. APA (Antheraea pernyi arylphorin) is an insect hexamerin that has two N-glycosylations at Asn(196) and Asn(344) respectively. The glycosylation of Asn(344) is critical for the folding process; however, glycosylation of Asn(196) is not. Interestingly, the N196-glycan (glycosylation of Asn(196)) remains in an immature form (Glc(1)Man(9)GlcNAc(2)). The mutation of Asn(196) to glutamine does not change the ecdysone-binding activity relative to that of the wild-type. In the present study, we determined the crystal structure of APA, and all sugar moieties of the N196-glycan were clearly observed in the electron-density map. Although the sugar moieties of the glycan generally have high structural flexibility, most sugar moieties of the N196-glycan were well organized in the deep cleft of the subunit interface and mediated many inter- and intra-subunit hydrogen bonds. Analytical ultracentrifugation and GdmCl (guanidinium chloride) unfolding experiments revealed that the presence of the N196-glycan was important for stabilizing the hexameric state and overall stability of APA respectively. Our results could provide a structural basis for studying not only other glycoproteins that carry an immature N-glycan, but also the structural role of N-glycans that are located in the deep cleft of a protein. | - |
dc.description.sponsorship | We thank S.M. Lee (Pusan National University, Busan, South Korea) and O.K. Cho (Korea Basic Science Institute) for the generous supply of insect haemolymph and mutant generation respectively. We also thank S. J. Park and N.Y. Oh (both at the Korea Basic Science Institute) for glycan analysis. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | PORTLAND PRESS LTD | - |
dc.subject | MOLAR HEAT-CAPACITY | - |
dc.subject | ENDOPLASMIC-RETICULUM | - |
dc.subject | LINKED OLIGOSACCHARIDES | - |
dc.subject | CRYSTAL-STRUCTURE | - |
dc.subject | AMINO-ACID | - |
dc.subject | GLYCOSYLATION | - |
dc.subject | GLYCANS | - |
dc.subject | STABILIZATION | - |
dc.subject | GLYCOPROTEIN | - |
dc.subject | HEMOCYANIN | - |
dc.title | The presence of monoglucosylated N196-glycan is important for the structural stability of storage protein, arylphorin | - |
dc.type | Article | - |
dc.identifier.wosid | 000267612200009 | - |
dc.identifier.scopusid | 2-s2.0-67650287954 | - |
dc.type.rims | ART | - |
dc.citation.volume | 421 | - |
dc.citation.beginningpage | 87 | - |
dc.citation.endingpage | 96 | - |
dc.citation.publicationname | BIOCHEMICAL JOURNAL | - |
dc.identifier.doi | 10.1042/BJ20082170 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, Jie-Oh | - |
dc.contributor.nonIdAuthor | Ryu, Kyoung-Seok | - |
dc.contributor.nonIdAuthor | Kwon, Taek Hun | - |
dc.contributor.nonIdAuthor | Choi, Han-Ho | - |
dc.contributor.nonIdAuthor | Park, Hong-Seog | - |
dc.contributor.nonIdAuthor | Hwang, Soo Kyung | - |
dc.contributor.nonIdAuthor | Lee, Zee-Won | - |
dc.contributor.nonIdAuthor | Lee, Kyung-Bok | - |
dc.contributor.nonIdAuthor | Han, Young Hyun | - |
dc.contributor.nonIdAuthor | Choi, Yun-Seok | - |
dc.contributor.nonIdAuthor | Jeon, Young Ho | - |
dc.contributor.nonIdAuthor | Cheong, Chaejoon | - |
dc.contributor.nonIdAuthor | Kim, Soohyun | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | arylphorin | - |
dc.subject.keywordAuthor | ecdysone | - |
dc.subject.keywordAuthor | glycosylation | - |
dc.subject.keywordAuthor | protein stability | - |
dc.subject.keywordAuthor | protein structure | - |
dc.subject.keywordAuthor | X-ray crystallography | - |
dc.subject.keywordPlus | MOLAR HEAT-CAPACITY | - |
dc.subject.keywordPlus | ENDOPLASMIC-RETICULUM | - |
dc.subject.keywordPlus | LINKED OLIGOSACCHARIDES | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | AMINO-ACID | - |
dc.subject.keywordPlus | GLYCOSYLATION | - |
dc.subject.keywordPlus | GLYCANS | - |
dc.subject.keywordPlus | STABILIZATION | - |
dc.subject.keywordPlus | GLYCOPROTEIN | - |
dc.subject.keywordPlus | HEMOCYANIN | - |
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