DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yun, Sang-Hoon | ko |
dc.contributor.author | Ji, Sang-Chun | ko |
dc.contributor.author | Jeon, Heung-Jin | ko |
dc.contributor.author | Wang, Xun | ko |
dc.contributor.author | Kim, Si-Wouk | ko |
dc.contributor.author | Bak, Geun-U | ko |
dc.contributor.author | Lee, Younghoon | ko |
dc.contributor.author | Lim, Heon M. | ko |
dc.date.accessioned | 2013-03-13T03:38:17Z | - |
dc.date.available | 2013-03-13T03:38:17Z | - |
dc.date.created | 2012-10-30 | - |
dc.date.created | 2012-10-30 | - |
dc.date.issued | 2012-09 | - |
dc.identifier.citation | PLOS ONE, v.7, no.9 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/10203/104384 | - |
dc.description.abstract | Cnu (an OriC-binding nucleoid protein) associates with H-NS. A variant of Cnu was identified as a key factor for filamentous growth of a wild-type Escherichia coli strain at 37 degrees C. This variant (CnuK9E) bears a substitution of a lysine to glutamic acid, causing a charge reversal in the first helix. The temperature-dependent filamentous growth of E. coli bearing CnuK9E could be reversed by either lowering the temperature to 25 degrees C or lowering the CnuK9E concentration in the cell. Gene expression analysis suggested that downregulation of dicA by CnuK9E causes a burst of dicB transcription, which, in turn, elicits filamentous growth. In vivo assays indicated that DicA transcriptionally activates its own gene, by binding to its operator in a temperature-dependent manner. The antagonizing effect of CnuK9E with H-NS on DNA-binding activity of DicA was stronger at 37 degrees C, presumably due to the lower operator binding of DicA at 37 degrees C. These data suggest that the temperature-dependent negative effect of CnuK9E on DicA binding plays a major role in filamentous growth. The C-terminus of DicA shows significant amino acid sequence similarity to the DNA-binding domains of RovA and SlyA, regulators of pathogenic genes in Yersinia and Salmonella, respectively, which also show better DNA-binding activity at 25 degrees C. | - |
dc.language | English | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.subject | NUCLEOID-ASSOCIATED PROTEIN | - |
dc.subject | DIVISION INHIBITION | - |
dc.subject | CELL-DIVISION | - |
dc.subject | YERSINIA-PSEUDOTUBERCULOSIS | - |
dc.subject | VIRF PROMOTER | - |
dc.subject | GENE DICB | - |
dc.subject | HHA | - |
dc.subject | VIRULENCE | - |
dc.subject | SEQUENCE | - |
dc.subject | REVEALS | - |
dc.title | The CnuK9E H-NS Complex Antagonizes DNA Binding of DicA and Leads to Temperature-Dependent Filamentous Growth in E-coli | - |
dc.type | Article | - |
dc.identifier.wosid | 000308788700098 | - |
dc.identifier.scopusid | 2-s2.0-84866369884 | - |
dc.type.rims | ART | - |
dc.citation.volume | 7 | - |
dc.citation.issue | 9 | - |
dc.citation.publicationname | PLOS ONE | - |
dc.identifier.doi | 10.1371/journal.pone.0045236 | - |
dc.contributor.localauthor | Lee, Younghoon | - |
dc.contributor.nonIdAuthor | Yun, Sang-Hoon | - |
dc.contributor.nonIdAuthor | Ji, Sang-Chun | - |
dc.contributor.nonIdAuthor | Jeon, Heung-Jin | - |
dc.contributor.nonIdAuthor | Wang, Xun | - |
dc.contributor.nonIdAuthor | Kim, Si-Wouk | - |
dc.contributor.nonIdAuthor | Lim, Heon M. | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | NUCLEOID-ASSOCIATED PROTEIN | - |
dc.subject.keywordPlus | DIVISION INHIBITION | - |
dc.subject.keywordPlus | CELL-DIVISION | - |
dc.subject.keywordPlus | YERSINIA-PSEUDOTUBERCULOSIS | - |
dc.subject.keywordPlus | VIRF PROMOTER | - |
dc.subject.keywordPlus | GENE DICB | - |
dc.subject.keywordPlus | HHA | - |
dc.subject.keywordPlus | VIRULENCE | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | REVEALS | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.