DC Field | Value | Language |
---|---|---|
dc.contributor.author | Min, Kyoungin | ko |
dc.contributor.author | Jo, Hunho | ko |
dc.contributor.author | Song, Kyungmi | ko |
dc.contributor.author | Cho, Minseon | ko |
dc.contributor.author | Chun, Yang-Sook | ko |
dc.contributor.author | Jon, Sangyong | ko |
dc.contributor.author | Kim, Won Jong | ko |
dc.contributor.author | Ban, Changill | ko |
dc.date.accessioned | 2013-03-12T20:51:19Z | - |
dc.date.available | 2013-03-12T20:51:19Z | - |
dc.date.created | 2012-07-09 | - |
dc.date.created | 2012-07-09 | - |
dc.date.issued | 2011-03 | - |
dc.identifier.citation | BIOMATERIALS, v.32, no.8, pp.2124 - 2132 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | http://hdl.handle.net/10203/103479 | - |
dc.description.abstract | We have designed a dual-aptamer complex specific to both prostate-specific membrane antigens (PSMA) (+) and (-) prostate cancer cells. In the complex, an A10 RNA aptamer targeting PSMA (+) cells and a DUP-1 peptide aptamer specific to PSMA (-) cells were conjugated through streptavidin. Doxorubicin-loaded onto the stem region of the A10 aptamer was delivered not only to PSMA (+) cells but to PSMA (-) cells, and eventually induced apoptosis in both types of prostate cancer cells. Cell death was monitored by measuring guanine concentration in cells using differential pulse voltammetry (DPV), a simple and rapid electrochemical method, and was further confirmed by directly observing cell morphologies cultured on the transparent indium tin oxide (ITO) glass electrode and checking their viabilities using a trypan blue assay. To investigate the in vivo application of the dual-aptamer system, both A10 and DUP-1 aptamers were immobilized on the surface of thermally cross-linked superparamagnetic iron oxide nanoparticles (TCL-SPION). Selective cell uptakes and effective drug delivery action of these probes were verified by Prussian blue staining and trypan blue staining, respectively. (C) 2010 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | IRON-OXIDE NANOPARTICLES | - |
dc.subject | TARGETED DRUG-DELIVERY | - |
dc.subject | IN-VIVO | - |
dc.subject | MEMBRANE ANTIGEN | - |
dc.subject | ELECTROCHEMICAL DETECTION | - |
dc.subject | CONTRAST AGENTS | - |
dc.subject | NUCLEIC-ACIDS | - |
dc.subject | DNA SENSORS | - |
dc.subject | THERAPY | - |
dc.subject | CELLS | - |
dc.title | Dual-aptamer-based delivery vehicle of doxorubicin to both PSMA (+) and PSMA (-) prostate cancers | - |
dc.type | Article | - |
dc.identifier.wosid | 000287061400013 | - |
dc.identifier.scopusid | 2-s2.0-78651419151 | - |
dc.type.rims | ART | - |
dc.citation.volume | 32 | - |
dc.citation.issue | 8 | - |
dc.citation.beginningpage | 2124 | - |
dc.citation.endingpage | 2132 | - |
dc.citation.publicationname | BIOMATERIALS | - |
dc.identifier.doi | 10.1016/j.biomaterials.2010.11.035 | - |
dc.contributor.localauthor | Jon, Sangyong | - |
dc.contributor.nonIdAuthor | Min, Kyoungin | - |
dc.contributor.nonIdAuthor | Jo, Hunho | - |
dc.contributor.nonIdAuthor | Song, Kyungmi | - |
dc.contributor.nonIdAuthor | Cho, Minseon | - |
dc.contributor.nonIdAuthor | Chun, Yang-Sook | - |
dc.contributor.nonIdAuthor | Kim, Won Jong | - |
dc.contributor.nonIdAuthor | Ban, Changill | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Drug delivery | - |
dc.subject.keywordAuthor | PSMA (+) and PSMA (-) prostate cancer cells | - |
dc.subject.keywordAuthor | A10 RNA aptamer | - |
dc.subject.keywordAuthor | DUP-1 peptide aptamer | - |
dc.subject.keywordAuthor | Differential pulse voltammetry | - |
dc.subject.keywordAuthor | Superparamagnetic iron oxide nanoparticle | - |
dc.subject.keywordPlus | IRON-OXIDE NANOPARTICLES | - |
dc.subject.keywordPlus | TARGETED DRUG-DELIVERY | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | MEMBRANE ANTIGEN | - |
dc.subject.keywordPlus | ELECTROCHEMICAL DETECTION | - |
dc.subject.keywordPlus | CONTRAST AGENTS | - |
dc.subject.keywordPlus | NUCLEIC-ACIDS | - |
dc.subject.keywordPlus | DNA SENSORS | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | CELLS | - |
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