Scopolamine-induced learning impairment reversed by physostigmine in zebrafish

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In this study, the effects of scopolamine, an acetylcholine muscarinic receptor antagonist, and physostigmine, an acetylcholinesterase inhibitor, on the learning ability and memory of zebrafish were evaluated using a passive avoidance response test. The zebrafish were trained to stay in a dark compartment to avoid a weight dropping into an acryl shuttle chamber with a central sliding door. The crossing time was increased significantly, from 30.7 +/- 40.8 s to 179.3 +/- 27.3 s in the training session and 179.9 +/- 28.0 s in the test session carried out 2 h later in the controls. When treatment with 200 mu M scopolamine was administered for 1 h prior to the training session, the crossing time did not increase. The scopolamine-induced learning deficit was ameliorated by pretreatment with 20 mu M physostigmine for 1 h prior to scopolamine treatment; the crossing time was similarly increased, as shown with the controls (60.9 +/- 11.5 s, 130.9 +/- 27.5 s, and 183.4 +/- 26.6 s in the training session and 108.1 +/- 23.9 s in the test session). When scopolamine treatment was administered after the training session, the crossing time in the test session was reduced significantly as compared to that noted in the third trial of the training session, which was also ameliorated by physostigmine pretreatment. These results show that scopolamine impairs both the acquisition of passive avoidance response and retention of the learned response, and that physostigmine rescues the amnesic effects of scopolamine in zebrafish. (C) 2010 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
Publisher
ELSEVIER IRELAND LTD
Issue Date
2010-06
Language
English
Article Type
Article
Keywords

ALZHEIMERS-DISEASE; CHOLINERGIC MODULATION; CHLORPYRIFOS EXPOSURE; MEMORY CONSOLIDATION; RATS; DONEPEZIL; DEFICITS; MODEL; HIPPOCAMPUS; NICOTINE

Citation

NEUROSCIENCE RESEARCH, v.67, pp.156 - 161

ISSN
0168-0102
DOI
10.1016/j.neures.2010.03.003
URI
http://hdl.handle.net/10203/103306
Appears in Collection
BS-Journal Papers(저널논문)
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