DC Field | Value | Language |
---|---|---|
dc.contributor.author | Umehara, Takuya | ko |
dc.contributor.author | Kim, Jihyo | ko |
dc.contributor.author | Lee, Sangsik | ko |
dc.contributor.author | Guo, Li-Tao | ko |
dc.contributor.author | Soell, Dieter | ko |
dc.contributor.author | Park, Hee-Sung | ko |
dc.date.accessioned | 2013-03-12T18:28:58Z | - |
dc.date.available | 2013-03-12T18:28:58Z | - |
dc.date.created | 2012-10-09 | - |
dc.date.created | 2012-10-09 | - |
dc.date.issued | 2012-03 | - |
dc.identifier.citation | FEBS LETTERS, v.586, no.6, pp.729 - 733 | - |
dc.identifier.issn | 0014-5793 | - |
dc.identifier.uri | http://hdl.handle.net/10203/103140 | - |
dc.description.abstract | Posttranslational modifications play a crucial role in modulating protein structure and function. Genetic incorporation of unnatural amino acids into a specific site of a protein facilitates the systematic study of protein modifications including acetylation. We here report the directed evolution of pyrrolysyl-tRNA synthetase (PylRS) from Methanosarcina mazei to create N-acetyl lysyl-tRNA synthetases (AcKRSs) using a new selection system based on the killing activity of the toxic ccdB gene product. The amino acid specificity of these and of published [1,2] AckRSs was tested in vitro and in vivo, and the enzyme-kinetic properties of the AckRSs were evaluated for the first time. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | GENETIC-CODE | - |
dc.subject | PROTEIN | - |
dc.subject | PYRROLYSINE | - |
dc.subject | MUTANTS | - |
dc.subject | DESIGN | - |
dc.title | N-Acetyl lysyl-tRNA synthetases evolved by a CcdB-based selection possess N-acetyl lysine specificity in vitro and in vivo | - |
dc.type | Article | - |
dc.identifier.wosid | 000301924600013 | - |
dc.identifier.scopusid | 2-s2.0-84862778261 | - |
dc.type.rims | ART | - |
dc.citation.volume | 586 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 729 | - |
dc.citation.endingpage | 733 | - |
dc.citation.publicationname | FEBS LETTERS | - |
dc.identifier.doi | 10.1016/j.febslet.2012.01.029 | - |
dc.contributor.localauthor | Park, Hee-Sung | - |
dc.contributor.nonIdAuthor | Umehara, Takuya | - |
dc.contributor.nonIdAuthor | Guo, Li-Tao | - |
dc.contributor.nonIdAuthor | Soell, Dieter | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | N-Acetyl lysyl-tRNA synthetase | - |
dc.subject.keywordAuthor | Directed evolution | - |
dc.subject.keywordAuthor | CcdB | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | GENETIC-CODE | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | PYRROLYSINE | - |
dc.subject.keywordPlus | MUTANTS | - |
dc.subject.keywordPlus | DESIGN | - |
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