DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Joon-Seok | ko |
dc.contributor.author | Joung, Hyou-Arm | ko |
dc.contributor.author | Kim, Min-Gon | ko |
dc.contributor.author | Park, Chan-Beum | ko |
dc.date.accessioned | 2013-03-12T08:56:58Z | - |
dc.date.available | 2013-03-12T08:56:58Z | - |
dc.date.created | 2012-08-02 | - |
dc.date.created | 2012-08-02 | - |
dc.date.issued | 2012-04 | - |
dc.identifier.citation | ACS NANO, v.6, no.4, pp.2978 - 2983 | - |
dc.identifier.issn | 1936-0851 | - |
dc.identifier.uri | http://hdl.handle.net/10203/101833 | - |
dc.description.abstract | We report on chemiluminescence resonance energy transfer (CRET) between graphene nanosheets and chemiluminescent donors. In contrast to fluorescence resonance energy transfer, CRET occurs via nonradiative dipole dipole transfer of energy from a chemiluminescent donor to a suitable acceptor molecule without an external excitation source. We designed a graphene-based CRET platform for homogeneous immunoassay of C-reactive protein (CRP), a key marker for human inflammation and cardiovascular diseases, using a luminol/hydrogen peroxide chemiluminescence (CL) reaction catalyzed by horseradish peroxidase. According to our results, anti-CRP antibody conjugated to graphene nanosheets enabled the capture of CRP at the concentration above 1.6 ngmL(-1). In the CRET platform, graphene played a key role as an energy acceptor, which was more efficient than graphene oxide, while luminol served as a donor to graphene, triggering the CRET phenomenon between luminol and graphene. The graphene-based CRET platform was successfully applied to the detection of CRP in human serum samples In the range observed during acute inflammatory stress. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | C-REACTIVE PROTEIN | - |
dc.subject | QUANTUM-DOTS | - |
dc.subject | OXIDE | - |
dc.subject | NANOCRYSTALS | - |
dc.subject | CELLS | - |
dc.title | Graphene-Based Chemiluminescence Resonance Energy Transfer for Homogeneous Immunoassay | - |
dc.type | Article | - |
dc.identifier.wosid | 000303099300014 | - |
dc.identifier.scopusid | 2-s2.0-84860371249 | - |
dc.type.rims | ART | - |
dc.citation.volume | 6 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 2978 | - |
dc.citation.endingpage | 2983 | - |
dc.citation.publicationname | ACS NANO | - |
dc.identifier.doi | 10.1021/nn300684d | - |
dc.contributor.localauthor | Park, Chan-Beum | - |
dc.contributor.nonIdAuthor | Joung, Hyou-Arm | - |
dc.contributor.nonIdAuthor | Kim, Min-Gon | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | graphene | - |
dc.subject.keywordAuthor | chemiluminescence | - |
dc.subject.keywordAuthor | CRET | - |
dc.subject.keywordAuthor | immunosensors | - |
dc.subject.keywordAuthor | C-reactive protein | - |
dc.subject.keywordPlus | C-REACTIVE PROTEIN | - |
dc.subject.keywordPlus | QUANTUM-DOTS | - |
dc.subject.keywordPlus | OXIDE | - |
dc.subject.keywordPlus | NANOCRYSTALS | - |
dc.subject.keywordPlus | CELLS | - |
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