DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Yeon-Gu | ko |
dc.contributor.author | Kim, Jee Yon | ko |
dc.contributor.author | Mohan, Chaya | ko |
dc.contributor.author | Lee, Gyun-Min | ko |
dc.date.accessioned | 2013-03-12T02:14:25Z | - |
dc.date.available | 2013-03-12T02:14:25Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2009-07 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOENGINEERING, v.103, no.4, pp.757 - 766 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | http://hdl.handle.net/10203/101064 | - |
dc.description.abstract | Upon nutrient deprivation during Culture, recombinant Chinese hamster ovary (rCHO) cells are subjected to two types of programmed cell death (PCD), apoptosis and autophagy. To investigate the effect of Bcl-x(L) overexpression on apoptosis and autophagy in rCHO cells, an erythropoietin (EPO)-producing rCHO cell line with regulated Bcl-x(L) overexpression (EPO-off-Bcl-x(L)) was established using the Tet-off system. The expression level of Bcl-x(L) in EPO-off-Bcl-x(L) Cells was tightly regulated by doxycycline in a dose-dependent manner. Bcl-x(L) overexpression enhanced cell viability and extended Culture longevity in batch culture. Upon nutrient depletion in the later stage of batch culture, Bcl-x(L) overexpression suppressed apoptosis by inhibiting the activation of caspase-3 and -7. Simultaneously, Bcl-x(L) overexpression also delayed autophagy, characterized by LC3-II accumulation. Immunoprecipitation analysis with a Flag-tagged Bcl-x(L) revealed that Bcl-x(L), interacts with Bax and Bak, essential mediators of caspase-dependent apoptosis, as well as with Beclin-1, an essential mediator of autophagy, and may inhibit their pro-cell death function. Taken together, it was found that Bcl-x(L) overexpression inhibits both apoptosis and autophagy in rCHO cell culture. Biotechnol. Bioeng. 2009003: 757-766. (c) 2009 Wiley Periodicals, Inc. | - |
dc.language | English | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.subject | BCL-2 FAMILY PROTEINS | - |
dc.subject | FED-BATCH CULTURES | - |
dc.subject | CHO-CELLS | - |
dc.subject | MAMMALIAN-CELLS | - |
dc.subject | DOWN-REGULATION | - |
dc.subject | DEATH | - |
dc.subject | INHIBITION | - |
dc.subject | BECLIN-1 | - |
dc.subject | ACTIVATION | - |
dc.subject | EXPRESSION | - |
dc.title | Effect of Bcl-x(L) Overexpression on Apoptosis and Autophagy in Recombinant Chinese Hamster Ovary Cells Under Nutrient-Deprived Condition | - |
dc.type | Article | - |
dc.identifier.wosid | 000268289500013 | - |
dc.identifier.scopusid | 2-s2.0-67650218292 | - |
dc.type.rims | ART | - |
dc.citation.volume | 103 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 757 | - |
dc.citation.endingpage | 766 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.identifier.doi | 10.1002/bit.22298 | - |
dc.contributor.localauthor | Lee, Gyun-Min | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | autophagy | - |
dc.subject.keywordAuthor | Bcl-x(L) | - |
dc.subject.keywordAuthor | rCHO cells | - |
dc.subject.keywordAuthor | inducible expression | - |
dc.subject.keywordPlus | BCL-2 FAMILY PROTEINS | - |
dc.subject.keywordPlus | FED-BATCH CULTURES | - |
dc.subject.keywordPlus | CHO-CELLS | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | DOWN-REGULATION | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | BECLIN-1 | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.