Kribbella ginsengisoli sp nov., isolated from soil of a ginseng field

Abstract

A Gram-positive, non-spore-forming, aerobic bacterium, designated Gsoil 001(T), was isolated from the soil of a ginseng field in Pocheon province, South Korea. On the basis of 16S rRNA gene sequence analysis, strain Gsoil 001(T) was shown to belong to the genus Kribbella. The closest phylogenetic relatives were Kribbella koreensis LM 161(T) (99.0% 16S rRNA gene sequence similarity), Kribbella flavida KACC 20248(T) (98.8%) and Kribbella alba (98.8%). Lower similarity (98.4-96.9%) was found with the other recognized species of the genus Kribbella. The major quinone was MK-9(H(4)), the major fatty acids were anteiso-C(15:0) and iso-C(16:0) and the major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The cell-wall peptidoglycan contained LL-2,6-diaminopimelic acid. These chemotaxonomic data and the high genomic DNA G + C content (66.3 mol%) supported the affiliation of strain Gsoil 001(T) to the genus Kribbella. The results of physiological and biochemical tests differentiated strain Gsoil 001T genotypically and phenotypically from the Kribbella species with validly published names. Therefore, this isolate represents a novel species, for which the name Kribbella ginsengisoli sp. nov. is proposed, with the type strain Gsoil 001(T) (=KCTC 19134(T) =DSM 17941(T)).