DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ahmad, Irshad | ko |
dc.contributor.author | Shim, Woo-Yong | ko |
dc.contributor.author | Jeon, Woo-Young | ko |
dc.contributor.author | Yoon, Byoung-Hoon | ko |
dc.contributor.author | Kim, Jung-Hoe | ko |
dc.date.accessioned | 2013-03-11T23:08:23Z | - |
dc.date.available | 2013-03-11T23:08:23Z | - |
dc.date.created | 2012-03-07 | - |
dc.date.created | 2012-03-07 | - |
dc.date.issued | 2012-01 | - |
dc.identifier.citation | BIOPROCESS AND BIOSYSTEMS ENGINEERING, v.35, pp.199 - 204 | - |
dc.identifier.issn | 1615-7591 | - |
dc.identifier.uri | http://hdl.handle.net/10203/100623 | - |
dc.description.abstract | The yeast Candida tropicalis produces xylitol, a natural, low-calorie sweetener whose metabolism does not require insulin, by catalytic activity of NADPH-dependent xylose reductase. The oxidative pentose phosphate pathway (PPP) is a major basis for NADPH biosynthesis in C. tropicalis. In order to increase xylitol production rate, xylitol dehydrogenase gene (XYL2)disrupted C. tropicalis strain BSXDH-3 was engineered to co-express zwf and gnd genes which, respectively encodes glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6-PGDH), under the control of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. NADPH-dependent xylitol production was higher in the engineered strain, termed "PP", than in BSXDH-3. In fermentation experiments using glycerol as a co-substrate with xylose, strain PP showed volumetric xylitol productivity of 1.25 g l(-1) h(-1), 21% higher than the rate (1.04 g l(-1) h(-1)) in BSXDH-3. This is the first report of increased metabolic flux toward PPP in C. tropicalis for NADPH regeneration and enhanced xylitol production. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.subject | RECOMBINANT SACCHAROMYCES-CEREVISIAE | - |
dc.subject | D-XYLOSE | - |
dc.subject | BIOTECHNOLOGICAL PRODUCTION | - |
dc.subject | DISRUPTED MUTANT | - |
dc.subject | DEHYDROGENASE | - |
dc.subject | FERMENTATION | - |
dc.subject | GUILLIERMONDII | - |
dc.subject | BIOREACTOR | - |
dc.subject | INCREASE | - |
dc.subject | YIELD | - |
dc.title | Enhancement of xylitol production in Candida tropicalis by co-expression of two genes involved in pentose phosphate pathway | - |
dc.type | Article | - |
dc.identifier.wosid | 000298799400028 | - |
dc.identifier.scopusid | 2-s2.0-84857452254 | - |
dc.type.rims | ART | - |
dc.citation.volume | 35 | - |
dc.citation.beginningpage | 199 | - |
dc.citation.endingpage | 204 | - |
dc.citation.publicationname | BIOPROCESS AND BIOSYSTEMS ENGINEERING | - |
dc.identifier.doi | 10.1007/s00449-011-0641-9 | - |
dc.contributor.localauthor | Kim, Jung-Hoe | - |
dc.type.journalArticle | Article; Proceedings Paper | - |
dc.subject.keywordAuthor | Xylitol | - |
dc.subject.keywordAuthor | Candida tropicalis | - |
dc.subject.keywordAuthor | Glucose-6-phosphate dehydrogenase | - |
dc.subject.keywordAuthor | 6-Phosphogluconate dehydrogenase | - |
dc.subject.keywordPlus | RECOMBINANT SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | D-XYLOSE | - |
dc.subject.keywordPlus | BIOTECHNOLOGICAL PRODUCTION | - |
dc.subject.keywordPlus | DISRUPTED MUTANT | - |
dc.subject.keywordPlus | DEHYDROGENASE | - |
dc.subject.keywordPlus | FERMENTATION | - |
dc.subject.keywordPlus | GUILLIERMONDII | - |
dc.subject.keywordPlus | BIOREACTOR | - |
dc.subject.keywordPlus | INCREASE | - |
dc.subject.keywordPlus | YIELD | - |
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