DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hong, Seong Cheol | ko |
dc.contributor.author | Chen, Hongxia | ko |
dc.contributor.author | Lee, Jaewook | ko |
dc.contributor.author | Park, Hye-Kyung | ko |
dc.contributor.author | Kim, Young Sik | ko |
dc.contributor.author | Shin, Hyun-Chul | ko |
dc.contributor.author | Kim, Cheol-Min | ko |
dc.contributor.author | Park, Tae Jung | ko |
dc.contributor.author | Lee, Seok Jae | ko |
dc.contributor.author | Koh, Kwangnak | ko |
dc.contributor.author | Kim, Hwa-Jung | ko |
dc.contributor.author | Chang, Chulhun L. | ko |
dc.contributor.author | Lee, Jaebeom | ko |
dc.date.accessioned | 2013-03-11T20:03:02Z | - |
dc.date.available | 2013-03-11T20:03:02Z | - |
dc.date.created | 2012-05-17 | - |
dc.date.created | 2012-05-17 | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | SENSORS AND ACTUATORS B-CHEMICAL, v.156, no.1, pp.271 - 275 | - |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | http://hdl.handle.net/10203/100126 | - |
dc.description.abstract | An antigen (Ag), CFP-10, found in tissue fluids of tuberculosis (TB) patients may be an ultimate candidate for use as a sensitive TB marker with a sensing method for early simplified diagnosis of TB. In this study, chemical and optical optimizations were carried out using novel immuno-materials for establishment of a self-assembled surface plasmon resonance (SPR) optical immunosensor system for detection of CFP-10, which is valuable for pre-clinical work, prior to conduct of massive clinical observations. For creation of a simple sensing interface, a monoclonal antibody (anti-CFP-10) was immobilized directly on a gold surface, followed by blocking with cystamine. Orientation and accessibility of anti-CFP-10 were assessed by the selective binding of CFP-10. Recent results indicate that the reusability of the sensor chip adopting the cystamine method was found to be preferable to other immobilization methods. A linear relationship was well correlated between SPR angle shift and CFP concentrations in the range from 100 ng mL(-1) to 1 mu g mL(-1). Modification of the SPR chip with antibody provides a simple experimental platform for investigation of isolated proteins under experimental conditions resembling those of their native environment. (C) 2011 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE SA | - |
dc.subject | SELF-ASSEMBLED MONOLAYERS | - |
dc.subject | MYCOBACTERIUM-TUBERCULOSIS | - |
dc.subject | INFECTION | - |
dc.subject | IMMUNOASSAY | - |
dc.subject | DIAGNOSIS | - |
dc.subject | PROTEIN | - |
dc.subject | ASSAY | - |
dc.subject | GOLD | - |
dc.title | Ultrasensitive immunosensing of tuberculosis CFP-10 based on SPR spectroscopy | - |
dc.type | Article | - |
dc.identifier.wosid | 000292058200039 | - |
dc.identifier.scopusid | 2-s2.0-79957850681 | - |
dc.type.rims | ART | - |
dc.citation.volume | 156 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 271 | - |
dc.citation.endingpage | 275 | - |
dc.citation.publicationname | SENSORS AND ACTUATORS B-CHEMICAL | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.nonIdAuthor | Hong, Seong Cheol | - |
dc.contributor.nonIdAuthor | Chen, Hongxia | - |
dc.contributor.nonIdAuthor | Lee, Jaewook | - |
dc.contributor.nonIdAuthor | Park, Hye-Kyung | - |
dc.contributor.nonIdAuthor | Kim, Young Sik | - |
dc.contributor.nonIdAuthor | Shin, Hyun-Chul | - |
dc.contributor.nonIdAuthor | Kim, Cheol-Min | - |
dc.contributor.nonIdAuthor | Lee, Seok Jae | - |
dc.contributor.nonIdAuthor | Koh, Kwangnak | - |
dc.contributor.nonIdAuthor | Kim, Hwa-Jung | - |
dc.contributor.nonIdAuthor | Chang, Chulhun L. | - |
dc.contributor.nonIdAuthor | Lee, Jaebeom | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | SELF-ASSEMBLED MONOLAYERS | - |
dc.subject.keywordPlus | MYCOBACTERIUM-TUBERCULOSIS | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | IMMUNOASSAY | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | GOLD | - |
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