Cationic Conjugated Polyelectrolytes-Triggered Conformational Change of Molecular Beacon Aptamer for Highly Sensitive and Selective Potassium Ion Detection

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We demonstrate highly sensitive and selective potassium ion detection against excess sodium ions in water, by modulating the interaction between the G-quadruplex-forming molecular beacon aptamer (MBA) and cationic conjugated polyelectrolyte (CPE). The K+-specific aptamer sequence in MBA is used as the molecular recognition element, and the high binding specificity of MBA for potassium ions offers selectivity against a range of metal ions. The hairpin-type MBA labeled with a fluorophore and quencher at both termini undergoes a conformational change (by complexation with CPEs) to either an open-chain form or a G-quadruplex in the absence or presence of K+ ions. Conformational changes of MBA as well as fluorescence (of the fluorophore in MBA) quenching or amplification via fluorescence resonance energy transfer from CPEs provide clear signal turn-off and -on in the presence or absence of ICE. The detection limit of the K+ assays is determined to be similar to 1.5 nM in the presence of 100 mM Na+ ions, which is similar to 3 orders of magnitude lower than those reported previously. The successful detection of 5'-adenosine triphosphate (ATP) with the MBA containing an ATP-specific aptamer sequence is also demonstrated using the same sensor scheme. The scheme reported herein is applicable to the detection of other kinds of G-rich aptamer-binding chemicals and biomolecules.
Publisher
AMER CHEMICAL SOC
Issue Date
2012-02
Language
English
Article Type
Article
Keywords

SWITCHING SIGNALING APTAMERS; G-QUADRUPLEX; LABEL-FREE; CHEMICAL SENSORS; OPTICAL PROBES; DNA APTAMER; FLUORESCENT; POLYMERS; WATER; RECOGNITION

Citation

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.134, no.6, pp.3133 - 3138

ISSN
0002-7863
DOI
10.1021/ja210360v
URI
http://hdl.handle.net/10203/98843
Appears in Collection
CH-Journal Papers(저널논문)
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