Cationic Conjugated Polyelectrolytes-Triggered Conformational Change of Molecular Beacon Aptamer for Highly Sensitive and Selective Potassium Ion Detection

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dc.contributor.authorKim, Boramko
dc.contributor.authorJung, In Hwanko
dc.contributor.authorKang, Mijeongko
dc.contributor.authorShim, Hong-Kuko
dc.contributor.authorWoo, Han Youngko
dc.date.accessioned2013-03-11T08:46:56Z-
dc.date.available2013-03-11T08:46:56Z-
dc.date.created2012-04-30-
dc.date.created2012-04-30-
dc.date.issued2012-02-
dc.identifier.citationJOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.134, no.6, pp.3133 - 3138-
dc.identifier.issn0002-7863-
dc.identifier.urihttp://hdl.handle.net/10203/98843-
dc.description.abstractWe demonstrate highly sensitive and selective potassium ion detection against excess sodium ions in water, by modulating the interaction between the G-quadruplex-forming molecular beacon aptamer (MBA) and cationic conjugated polyelectrolyte (CPE). The K+-specific aptamer sequence in MBA is used as the molecular recognition element, and the high binding specificity of MBA for potassium ions offers selectivity against a range of metal ions. The hairpin-type MBA labeled with a fluorophore and quencher at both termini undergoes a conformational change (by complexation with CPEs) to either an open-chain form or a G-quadruplex in the absence or presence of K+ ions. Conformational changes of MBA as well as fluorescence (of the fluorophore in MBA) quenching or amplification via fluorescence resonance energy transfer from CPEs provide clear signal turn-off and -on in the presence or absence of ICE. The detection limit of the K+ assays is determined to be similar to 1.5 nM in the presence of 100 mM Na+ ions, which is similar to 3 orders of magnitude lower than those reported previously. The successful detection of 5'-adenosine triphosphate (ATP) with the MBA containing an ATP-specific aptamer sequence is also demonstrated using the same sensor scheme. The scheme reported herein is applicable to the detection of other kinds of G-rich aptamer-binding chemicals and biomolecules.-
dc.languageEnglish-
dc.publisherAMER CHEMICAL SOC-
dc.subjectSWITCHING SIGNALING APTAMERS-
dc.subjectG-QUADRUPLEX-
dc.subjectLABEL-FREE-
dc.subjectCHEMICAL SENSORS-
dc.subjectOPTICAL PROBES-
dc.subjectDNA APTAMER-
dc.subjectFLUORESCENT-
dc.subjectPOLYMERS-
dc.subjectWATER-
dc.subjectRECOGNITION-
dc.titleCationic Conjugated Polyelectrolytes-Triggered Conformational Change of Molecular Beacon Aptamer for Highly Sensitive and Selective Potassium Ion Detection-
dc.typeArticle-
dc.identifier.wosid000301161500053-
dc.identifier.scopusid2-s2.0-84863116181-
dc.type.rimsART-
dc.citation.volume134-
dc.citation.issue6-
dc.citation.beginningpage3133-
dc.citation.endingpage3138-
dc.citation.publicationnameJOURNAL OF THE AMERICAN CHEMICAL SOCIETY-
dc.identifier.doi10.1021/ja210360v-
dc.contributor.localauthorShim, Hong-Ku-
dc.contributor.nonIdAuthorKim, Boram-
dc.contributor.nonIdAuthorKang, Mijeong-
dc.contributor.nonIdAuthorWoo, Han Young-
dc.type.journalArticleArticle-
dc.subject.keywordPlusSWITCHING SIGNALING APTAMERS-
dc.subject.keywordPlusG-QUADRUPLEX-
dc.subject.keywordPlusLABEL-FREE-
dc.subject.keywordPlusCHEMICAL SENSORS-
dc.subject.keywordPlusOPTICAL PROBES-
dc.subject.keywordPlusDNA APTAMER-
dc.subject.keywordPlusFLUORESCENT-
dc.subject.keywordPlusPOLYMERS-
dc.subject.keywordPlusWATER-
dc.subject.keywordPlusRECOGNITION-
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