DNA capturing machinery through spore-displayed proteins

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Aims: The purpose of this study was to develop a general method for the facile development of a new DNA biosensor which utilizes streptavidin-displayed spores as a molecular machinery. Methods and Results: Fluorescence spectroscopy was used as a monitoring tool for the streptavidin displayed on the surface of Bacillus thuringiensis spores and as a diagnosis method for DNA detection. As a proof-of-concept, four pathogenic bacteria including Pseudomonas aeruginosa, Acinetobacter baumannii, Escherichia coli and Klebsiella pneumonia were used for the detection of pathogenic species. In addition, a set of mutant variants of Wilson's disease were also used for the detection of single nucleotide polymorphism (SNP) in this system. Conclusions: This strategy, utilizing streptavidin-displayed spores, is capable of capturing DNA targets for the detection of pathogenic bacteria and for mutation analysis in Wilson's disease. Significance and Impact of the Study: This approach could be useful as a simple platform for developing sensitive spore-based biosensors for any desired DNA targets in diagnostic applications.
Publisher
WILEY-BLACKWELL
Issue Date
2011-10
Language
English
Article Type
Article
Keywords

BACTERIAL SURFACE DISPLAY; ESCHERICHIA-COLI; WILSON-DISEASE; CELL-SURFACE; MUTATIONS; SYSTEM; STREPTAVIDIN; FREQUENCY; VECTOR; GENE

Citation

LETTERS IN APPLIED MICROBIOLOGY, v.53, no.4, pp.445 - 451

ISSN
0266-8254
URI
http://hdl.handle.net/10203/98015
Appears in Collection
CBE-Journal Papers(저널논문)
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