DNA capturing machinery through spore-displayed proteins

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 265
  • Download : 0
DC FieldValueLanguage
dc.contributor.authorPark, TJko
dc.contributor.authorLee, SJko
dc.contributor.authorPan, JGko
dc.contributor.authorJung, HCko
dc.contributor.authorPark, JYko
dc.contributor.authorPark, JPko
dc.contributor.authorLee, SangYupko
dc.date.accessioned2013-03-11T02:13:24Z-
dc.date.available2013-03-11T02:13:24Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2011-10-
dc.identifier.citationLETTERS IN APPLIED MICROBIOLOGY, v.53, no.4, pp.445 - 451-
dc.identifier.issn0266-8254-
dc.identifier.urihttp://hdl.handle.net/10203/98015-
dc.description.abstractAims: The purpose of this study was to develop a general method for the facile development of a new DNA biosensor which utilizes streptavidin-displayed spores as a molecular machinery. Methods and Results: Fluorescence spectroscopy was used as a monitoring tool for the streptavidin displayed on the surface of Bacillus thuringiensis spores and as a diagnosis method for DNA detection. As a proof-of-concept, four pathogenic bacteria including Pseudomonas aeruginosa, Acinetobacter baumannii, Escherichia coli and Klebsiella pneumonia were used for the detection of pathogenic species. In addition, a set of mutant variants of Wilson's disease were also used for the detection of single nucleotide polymorphism (SNP) in this system. Conclusions: This strategy, utilizing streptavidin-displayed spores, is capable of capturing DNA targets for the detection of pathogenic bacteria and for mutation analysis in Wilson's disease. Significance and Impact of the Study: This approach could be useful as a simple platform for developing sensitive spore-based biosensors for any desired DNA targets in diagnostic applications.-
dc.languageEnglish-
dc.publisherWILEY-BLACKWELL-
dc.subjectBACTERIAL SURFACE DISPLAY-
dc.subjectESCHERICHIA-COLI-
dc.subjectWILSON-DISEASE-
dc.subjectCELL-SURFACE-
dc.subjectMUTATIONS-
dc.subjectSYSTEM-
dc.subjectSTREPTAVIDIN-
dc.subjectFREQUENCY-
dc.subjectVECTOR-
dc.subjectGENE-
dc.titleDNA capturing machinery through spore-displayed proteins-
dc.typeArticle-
dc.identifier.wosid000295095600009-
dc.identifier.scopusid2-s2.0-80052639552-
dc.type.rimsART-
dc.citation.volume53-
dc.citation.issue4-
dc.citation.beginningpage445-
dc.citation.endingpage451-
dc.citation.publicationnameLETTERS IN APPLIED MICROBIOLOGY-
dc.contributor.localauthorLee, SangYup-
dc.contributor.nonIdAuthorLee, SJ-
dc.contributor.nonIdAuthorPan, JG-
dc.contributor.nonIdAuthorJung, HC-
dc.contributor.nonIdAuthorPark, JY-
dc.contributor.nonIdAuthorPark, JP-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorpathogenic bacteria-
dc.subject.keywordAuthorsingle nucleotide polymorphism detection-
dc.subject.keywordAuthorspore display-
dc.subject.keywordAuthorstreptavidin-
dc.subject.keywordAuthorWilson&apos-
dc.subject.keywordAuthors disease-
dc.subject.keywordPlusBACTERIAL SURFACE DISPLAY-
dc.subject.keywordPlusESCHERICHIA-COLI-
dc.subject.keywordPlusWILSON-DISEASE-
dc.subject.keywordPlusCELL-SURFACE-
dc.subject.keywordPlusMUTATIONS-
dc.subject.keywordPlusSYSTEM-
dc.subject.keywordPlusSTREPTAVIDIN-
dc.subject.keywordPlusFREQUENCY-
dc.subject.keywordPlusVECTOR-
dc.subject.keywordPlusGENE-
Appears in Collection
CBE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0