Sphingomonas soli sp nov., a beta-glucosidase-producing bacterium in the family Sphingomonadaceae in the alpha-4 subgroup of the Proteobacteria

Abstract

Strain T5-04(T), a Gram-negative, non-spore-forming, rod-shaped bacterium, was isolated from soil of a ginseng field in South Korea and characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain T5-04T belongs to the U-4 subgroup of the Proteobacteria, and the highest degrees of sequence similarity determined were to Sphingomonas asaccharolytica IFO 10564(T) (97.5%), Sphingomonas koreensis JSS26(T) (97(.)1%), Sphingomonas mali IFO 15500(T) (96(.)7%) and Sphingomonas pruni IFO 15498(T) (96(.)6%). Chemotaxonomic data revealed that strain T5-04(T) possesses ubiquinone Q-10 predominantly, C-18:1 as the predominant fatty acid and sphingoglycolipids, all of which corroborate its assignment to the genus Sphingomonas. The results of DNA-DNA hybridization and physiological and biochemical tests clearly demonstrated that strain T5-04(T) represents a distinct species. Based on polyphasic; evidence, T5-04(T) (= KCTC 12210(T) NBRC 10080(T)= IAM 15213(T)) should be classified as the type strain of a novel Sphingomonas species, for which the name Sphingomonas soli sp. nov. is proposed.