Production of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat

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We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 mu g/ml in transgenic goat milk. Its biological activity was equivalent to recombinant human G-CSF expressed from Chinese hamster ovary (CHO) cell when assayed using in vitro HL-60 cell proliferation. Human G-CSF from transgenic goat milk increased the total number of white blood cells in C57BL/6N mice with leucopenia induced by cyclophosphamide (CPA). The secreted human G-CSF was glycosylated although the degree of O-glycosylation was lower compared to CHO cell-derived human G-CSF.
Publisher
KLUWER ACADEMIC PUBL
Issue Date
2000-06
Language
English
Article Type
Article
Keywords

HIGH-LEVEL EXPRESSION; MAMMARY-GLAND; BETA-LACTOGLOBULIN; MICE; GENE; PROTEIN; SHEEP; CELLS; CSF; PURIFICATION

Citation

TRANSGENIC RESEARCH, v.9, no.3, pp.215 - 222

ISSN
0962-8819
URI
http://hdl.handle.net/10203/74202
Appears in Collection
MSE-Journal Papers(저널논문)
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