DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ko, JH | ko |
dc.contributor.author | Lee, CS | ko |
dc.contributor.author | Kim, KH | ko |
dc.contributor.author | Pang, MG | ko |
dc.contributor.author | Koo, JS | ko |
dc.contributor.author | Fang, NZ | ko |
dc.contributor.author | Koo, DB | ko |
dc.contributor.author | Oh, KB | ko |
dc.contributor.author | Youn, WS | ko |
dc.contributor.author | Zheng, GD | ko |
dc.contributor.author | Park, JS | ko |
dc.contributor.author | Kim, SJ | ko |
dc.contributor.author | Han, YM | ko |
dc.contributor.author | Choi, IY | ko |
dc.contributor.author | Lim, J | ko |
dc.contributor.author | Shin, ST | ko |
dc.contributor.author | Jin, SW | ko |
dc.contributor.author | Lee, KK | ko |
dc.contributor.author | Yoo, Ook-Joon | ko |
dc.date.accessioned | 2013-03-02T15:32:37Z | - |
dc.date.available | 2013-03-02T15:32:37Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2000-06 | - |
dc.identifier.citation | TRANSGENIC RESEARCH, v.9, no.3, pp.215 - 222 | - |
dc.identifier.issn | 0962-8819 | - |
dc.identifier.uri | http://hdl.handle.net/10203/74202 | - |
dc.description.abstract | We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 mu g/ml in transgenic goat milk. Its biological activity was equivalent to recombinant human G-CSF expressed from Chinese hamster ovary (CHO) cell when assayed using in vitro HL-60 cell proliferation. Human G-CSF from transgenic goat milk increased the total number of white blood cells in C57BL/6N mice with leucopenia induced by cyclophosphamide (CPA). The secreted human G-CSF was glycosylated although the degree of O-glycosylation was lower compared to CHO cell-derived human G-CSF. | - |
dc.language | English | - |
dc.publisher | KLUWER ACADEMIC PUBL | - |
dc.subject | HIGH-LEVEL EXPRESSION | - |
dc.subject | MAMMARY-GLAND | - |
dc.subject | BETA-LACTOGLOBULIN | - |
dc.subject | MICE | - |
dc.subject | GENE | - |
dc.subject | PROTEIN | - |
dc.subject | SHEEP | - |
dc.subject | CELLS | - |
dc.subject | CSF | - |
dc.subject | PURIFICATION | - |
dc.title | Production of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat | - |
dc.type | Article | - |
dc.identifier.wosid | 000089139300007 | - |
dc.identifier.scopusid | 2-s2.0-0033800863 | - |
dc.type.rims | ART | - |
dc.citation.volume | 9 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 215 | - |
dc.citation.endingpage | 222 | - |
dc.citation.publicationname | TRANSGENIC RESEARCH | - |
dc.contributor.localauthor | Yoo, Ook-Joon | - |
dc.contributor.nonIdAuthor | Ko, JH | - |
dc.contributor.nonIdAuthor | Lee, CS | - |
dc.contributor.nonIdAuthor | Kim, KH | - |
dc.contributor.nonIdAuthor | Pang, MG | - |
dc.contributor.nonIdAuthor | Koo, JS | - |
dc.contributor.nonIdAuthor | Fang, NZ | - |
dc.contributor.nonIdAuthor | Koo, DB | - |
dc.contributor.nonIdAuthor | Oh, KB | - |
dc.contributor.nonIdAuthor | Youn, WS | - |
dc.contributor.nonIdAuthor | Zheng, GD | - |
dc.contributor.nonIdAuthor | Park, JS | - |
dc.contributor.nonIdAuthor | Kim, SJ | - |
dc.contributor.nonIdAuthor | Han, YM | - |
dc.contributor.nonIdAuthor | Choi, IY | - |
dc.contributor.nonIdAuthor | Lim, J | - |
dc.contributor.nonIdAuthor | Shin, ST | - |
dc.contributor.nonIdAuthor | Jin, SW | - |
dc.contributor.nonIdAuthor | Lee, KK | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | biological activity | - |
dc.subject.keywordAuthor | bioreactor | - |
dc.subject.keywordAuthor | G-CSF | - |
dc.subject.keywordAuthor | O-glycosylation | - |
dc.subject.keywordAuthor | transgenic goat | - |
dc.subject.keywordPlus | HIGH-LEVEL EXPRESSION | - |
dc.subject.keywordPlus | MAMMARY-GLAND | - |
dc.subject.keywordPlus | BETA-LACTOGLOBULIN | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | SHEEP | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | CSF | - |
dc.subject.keywordPlus | PURIFICATION | - |
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