Production of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat

Cited 35 time in webofscience Cited 0 time in scopus
  • Hit : 233
  • Download : 0
DC FieldValueLanguage
dc.contributor.authorKo, JHko
dc.contributor.authorLee, CSko
dc.contributor.authorKim, KHko
dc.contributor.authorPang, MGko
dc.contributor.authorKoo, JSko
dc.contributor.authorFang, NZko
dc.contributor.authorKoo, DBko
dc.contributor.authorOh, KBko
dc.contributor.authorYoun, WSko
dc.contributor.authorZheng, GDko
dc.contributor.authorPark, JSko
dc.contributor.authorKim, SJko
dc.contributor.authorHan, YMko
dc.contributor.authorChoi, IYko
dc.contributor.authorLim, Jko
dc.contributor.authorShin, STko
dc.contributor.authorJin, SWko
dc.contributor.authorLee, KKko
dc.contributor.authorYoo, Ook-Joonko
dc.date.accessioned2013-03-02T15:32:37Z-
dc.date.available2013-03-02T15:32:37Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2000-06-
dc.identifier.citationTRANSGENIC RESEARCH, v.9, no.3, pp.215 - 222-
dc.identifier.issn0962-8819-
dc.identifier.urihttp://hdl.handle.net/10203/74202-
dc.description.abstractWe have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 mu g/ml in transgenic goat milk. Its biological activity was equivalent to recombinant human G-CSF expressed from Chinese hamster ovary (CHO) cell when assayed using in vitro HL-60 cell proliferation. Human G-CSF from transgenic goat milk increased the total number of white blood cells in C57BL/6N mice with leucopenia induced by cyclophosphamide (CPA). The secreted human G-CSF was glycosylated although the degree of O-glycosylation was lower compared to CHO cell-derived human G-CSF.-
dc.languageEnglish-
dc.publisherKLUWER ACADEMIC PUBL-
dc.subjectHIGH-LEVEL EXPRESSION-
dc.subjectMAMMARY-GLAND-
dc.subjectBETA-LACTOGLOBULIN-
dc.subjectMICE-
dc.subjectGENE-
dc.subjectPROTEIN-
dc.subjectSHEEP-
dc.subjectCELLS-
dc.subjectCSF-
dc.subjectPURIFICATION-
dc.titleProduction of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat-
dc.typeArticle-
dc.identifier.wosid000089139300007-
dc.identifier.scopusid2-s2.0-0033800863-
dc.type.rimsART-
dc.citation.volume9-
dc.citation.issue3-
dc.citation.beginningpage215-
dc.citation.endingpage222-
dc.citation.publicationnameTRANSGENIC RESEARCH-
dc.contributor.localauthorYoo, Ook-Joon-
dc.contributor.nonIdAuthorKo, JH-
dc.contributor.nonIdAuthorLee, CS-
dc.contributor.nonIdAuthorKim, KH-
dc.contributor.nonIdAuthorPang, MG-
dc.contributor.nonIdAuthorKoo, JS-
dc.contributor.nonIdAuthorFang, NZ-
dc.contributor.nonIdAuthorKoo, DB-
dc.contributor.nonIdAuthorOh, KB-
dc.contributor.nonIdAuthorYoun, WS-
dc.contributor.nonIdAuthorZheng, GD-
dc.contributor.nonIdAuthorPark, JS-
dc.contributor.nonIdAuthorKim, SJ-
dc.contributor.nonIdAuthorHan, YM-
dc.contributor.nonIdAuthorChoi, IY-
dc.contributor.nonIdAuthorLim, J-
dc.contributor.nonIdAuthorShin, ST-
dc.contributor.nonIdAuthorJin, SW-
dc.contributor.nonIdAuthorLee, KK-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorbiological activity-
dc.subject.keywordAuthorbioreactor-
dc.subject.keywordAuthorG-CSF-
dc.subject.keywordAuthorO-glycosylation-
dc.subject.keywordAuthortransgenic goat-
dc.subject.keywordPlusHIGH-LEVEL EXPRESSION-
dc.subject.keywordPlusMAMMARY-GLAND-
dc.subject.keywordPlusBETA-LACTOGLOBULIN-
dc.subject.keywordPlusMICE-
dc.subject.keywordPlusGENE-
dc.subject.keywordPlusPROTEIN-
dc.subject.keywordPlusSHEEP-
dc.subject.keywordPlusCELLS-
dc.subject.keywordPlusCSF-
dc.subject.keywordPlusPURIFICATION-
Appears in Collection
MSE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 35 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0