DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoon, JH | ko |
dc.contributor.author | Rhee, Joon Shick | ko |
dc.date.accessioned | 2013-02-27T11:14:10Z | - |
dc.date.available | 2013-02-27T11:14:10Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2000-08 | - |
dc.identifier.citation | CARBOHYDRATE RESEARCH, v.327, no.4, pp.377 - 383 | - |
dc.identifier.issn | 0008-6215 | - |
dc.identifier.uri | http://hdl.handle.net/10203/68174 | - |
dc.description.abstract | In the presence of beta-galactosidase from Bifidobacterium bifidum, N-acetyllactosamine was synthesized in significantly enhanced yield compared with earlier routes. Different proportions of the (1-->4)- and (1-->6)-linked forms were obtained depending on the choice of enzyme and reaction conditions, viz. the nature of added organic co-solvent (20-80% of 2-ethoxy ethyl ether, trimethyl phosphate, or acetone). The beta-(1 --> 4)-linked disaccharide was the major product and the beta-(1-->6)-linked disaccharide was the minor product. With beta-galactosidases from P. multicolor, A. oryzae, B. longum the beta-(1 --> 6) linkage was exclusively synthesized. Procedures for optimising the yield of N-acetyllactosamine are discussed. An immobilized enzyme on a nylon powder column was used for the efficient recycling of enzyme and synthesizing the disaccharide. (C) 2000 Elsevier Science Ltd. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | BETA-GALACTOSIDASES | - |
dc.subject | OLIGOSACCHARIDES | - |
dc.subject | DISACCHARIDES | - |
dc.subject | GLYCOSIDASES | - |
dc.subject | DERIVATIVES | - |
dc.subject | ORIGINS | - |
dc.title | The efficient enzymatic synthesis of N-acetyllactosamine in an organic co-solvent | - |
dc.type | Article | - |
dc.identifier.wosid | 000088527100003 | - |
dc.type.rims | ART | - |
dc.citation.volume | 327 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 377 | - |
dc.citation.endingpage | 383 | - |
dc.citation.publicationname | CARBOHYDRATE RESEARCH | - |
dc.identifier.doi | 10.1016/S0008-6215(00)00063-X | - |
dc.contributor.nonIdAuthor | Yoon, JH | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | immobilized beta-galactosidase | - |
dc.subject.keywordAuthor | Bifidobacterium bifidum | - |
dc.subject.keywordAuthor | N-acetyllactosamine | - |
dc.subject.keywordAuthor | organic co-solvents | - |
dc.subject.keywordAuthor | nylon powder | - |
dc.subject.keywordPlus | BETA-GALACTOSIDASES | - |
dc.subject.keywordPlus | OLIGOSACCHARIDES | - |
dc.subject.keywordPlus | DISACCHARIDES | - |
dc.subject.keywordPlus | GLYCOSIDASES | - |
dc.subject.keywordPlus | DERIVATIVES | - |
dc.subject.keywordPlus | ORIGINS | - |
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