A graphene oxide-based tool-kit capable of characterizing and classifying exonuclease activities

Abstract

Enzyme kinetics and classification are key topics in modern biochemistry. Here, we have developed a simple, rapid, and quantitative assay via a terminally capped DNA probe, reaction-specific-labeling and the fluorescence-quenching capability of graphene-oxide. Our technique provides not only characterization of kinetic information but also the immediate classification of an exonuclease without needing to design a set of DNA substrates.