Fluorescence polarization-based detection of cancer-related mutations using target-initiated rolling circle amplification

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We devised a new method to detect cancer-related mutations based on target-initiated rolling circle amplification in combination with fluorescence polarization. We then applied this method to identify the presence of KRAS G13D and G12D, two of the most frequent mutations found in colorectal cancer patients, demonstrating high sensitivity and specificity.
Publisher
ROYAL SOC CHEMISTRY
Issue Date
2019-07
Language
English
Article Type
Article
Citation

ANALYST, v.144, no.14, pp.4149 - 4152

ISSN
0003-2654
DOI
10.1039/c9an00429g
URI
http://hdl.handle.net/10203/263876
Appears in Collection
CBE-Journal Papers(저널논문)
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