Production of bioactive ginsenoside Rg3(S) and compound K using recombinant Lactococcus lactis

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Background: Ginsenoside Rg3(S) and compound K (C-K) are pharmacologically active components of ginseng that promote human health and improve quality of life. The aim of this study was to produce Rg3(S) and C-K from ginseng extract using recombinant Lactococcus lactis. Methods: L. lactis subsp. cremoris NZ9000 (L. lactis NZ9000), which harbors beta-glucosidase genes (BglPm and BglBX10) from Paenibacillus mucilaginosus and Flavobacterium johnsoniae, respectively, was reacted with ginseng extract (protopanaxadiol-type ginsenoside mixture). Results: Crude enzyme activity of BglBX10 values comprised 0.001 unit/mL and 0.003 unit/mL in uninduced and induced preparations, respectively. When whole cells of L. lactis harboring pNZBglBX10 were treated with ginseng extract, after permeabilization of cells by xylene, Rb1 and Rd were converted into Rg3(S) with a conversion yield of 61%. C-K was also produced by sequential reactions of the permeabilized cells harboring each pNZBgl and pNZBglBX10, resulting in a 70% maximum conversion yield. Conclusion: This study demonstrates that the lactic acid bacteria having specific beta-glucosidase activity can be used to enhance the health benefits of Panax ginseng in either fermented foods or bioconversion processes. (C) 2017 The Korean Society of Ginseng, Published by Elsevier Korea LLC.
Publisher
KOREAN SOC GINSENG
Issue Date
2018-10
Language
English
Article Type
Article
Citation

JOURNAL OF GINSENG RESEARCH, v.42, no.4, pp.412 - 418

ISSN
1226-8453
DOI
10.1016/j.jgr.2017.04.007
URI
http://hdl.handle.net/10203/246546
Appears in Collection
BS-Journal Papers(저널논문)
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