Correlative three-dimensional fluorescence and refractive index tomography: bridging the gap between molecular specificity and quantitative bioimaging

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Optical diffraction tomography (ODT) provides label-free three-dimensional (3D) refractive index (RI) measurement of biological samples. However, due to the nature of the RI values of biological specimens, ODT has limited access to molecular specific information. Here, we present an optical setup combining ODT with three-channel 3D fluorescence microscopy, to enhance the molecular specificity of the 3D RI measurement. The 3D RI distribution and 3D deconvoluted fluorescence images of HeLa cells and NIH-3T3 cells are measured, and the cross-correlative analysis between RI and fluorescence of live cells are presented.
Publisher
OPTICAL SOC AMER
Issue Date
2017-11
Language
English
Article Type
Article
Citation

BIOMEDICAL OPTICS EXPRESS, v.8, no.12, pp.5688 - 5697

ISSN
2156-7085
DOI
10.1364/BOE.8.005688
URI
http://hdl.handle.net/10203/238827
Appears in Collection
BS-Journal Papers(저널논문)PH-Journal Papers(저널논문)
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