DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Suhyung | ko |
dc.contributor.author | Cho, Yoo-Bok | ko |
dc.contributor.author | Kang, Taek Jin | ko |
dc.contributor.author | Kim, Sun Chang | ko |
dc.contributor.author | Palsson, Bernhard | ko |
dc.contributor.author | Cho, Byung-Kwan | ko |
dc.date.accessioned | 2015-06-25T06:28:31Z | - |
dc.date.available | 2015-06-25T06:28:31Z | - |
dc.date.created | 2015-05-13 | - |
dc.date.created | 2015-05-13 | - |
dc.date.created | 2015-05-13 | - |
dc.date.issued | 2015-03 | - |
dc.identifier.citation | NUCLEIC ACIDS RESEARCH, v.43, no.6, pp.3079 - 3088 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | http://hdl.handle.net/10203/199071 | - |
dc.description.abstract | DNA-binding motifs that are recognized by transcription factors (TFs) have been well studied; however, challenges remain in determining the in vivo architecture of TF-DNA complexes on a genome-scale. Here, we determined the in vivo architecture of Escherichia coli arginine repressor (ArgR)-DNA complexes using high-throughput sequencing of exonuclease-treated chromatin-immunoprecipitated DNA (ChIP-exo). The ChIP-exo has a unique peak-pair pattern indicating 5' and 3' ends of ArgR-binding region. We identified 62 ArgR-binding loci, which were classified into three groups, comprising single, double and triple peak-pairs. Each peak-pair has a unique 93 base pair (bp)-long (+/- 2 bp) ArgR-binding sequence containing two ARG boxes (39 bp) and residual sequences. Moreover, the three ArgR-binding modes defined by the position of the two ARG boxes indicate that DNA bends centered between the pair of ARG boxes facilitate the non-specific contacts between ArgR subunits and the residual sequences. Additionally, our approach may also reveal other fundamental structural features of TF-DNA interactions that have implications for studying genome-scale transcriptional regulatory networks. | - |
dc.language | English | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.title | The architecture of ArgR-DNA complexes at the genome-scale in Escherichia coli | - |
dc.type | Article | - |
dc.identifier.wosid | 000354719300016 | - |
dc.identifier.scopusid | 2-s2.0-84941746034 | - |
dc.type.rims | ART | - |
dc.citation.volume | 43 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 3079 | - |
dc.citation.endingpage | 3088 | - |
dc.citation.publicationname | NUCLEIC ACIDS RESEARCH | - |
dc.identifier.doi | 10.1093/nar/gkv150 | - |
dc.contributor.localauthor | Kim, Sun Chang | - |
dc.contributor.localauthor | Cho, Byung-Kwan | - |
dc.contributor.nonIdAuthor | Cho, Suhyung | - |
dc.contributor.nonIdAuthor | Cho, Yoo-Bok | - |
dc.contributor.nonIdAuthor | Kang, Taek Jin | - |
dc.contributor.nonIdAuthor | Palsson, Bernhard | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | HEXAMERIC ARGININE REPRESSOR | - |
dc.subject.keywordPlus | BINDING DOMAIN | - |
dc.subject.keywordPlus | OPERATOR INTERACTIONS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | RNA-POLYMERASE | - |
dc.subject.keywordPlus | H-NS | - |
dc.subject.keywordPlus | PROMOTERS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | K-12 | - |
dc.subject.keywordPlus | REGULON | - |
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