Multi-stage continuous high cell density culture systems: A review

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A multi-stage continuous high cell density culture (MSC-HCDC) system makes it possible to achieve high productivity together with high product titer of many bioproducts. For long-term continuous operation of MSC-HCDC systems, the cell retention time and hydraulic retention time must be decoupled and strains (bacteria, yeast, plant, and animal cells) must be stable. MSC-HCDC systems are suitable for low-value high-volume extracellular products such as fuel ethanol, lactic acid or volatile fatty acids, and high-value products such as monoclonal antibodies as well as intracellular products such as polyhydroxybutyric acid (PHB), microbial lipids or a number of therapeutics. Better understanding of the fermentation kinetics of a specific product and reliable high-density culture methods for the product-generating microorganisms will facilitate timely industrialization of MSC-HCDC systems for products that are currently obtained in fed-batch bioreactors.
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Issue Date
2014-03
Language
English
Article Type
Review
Keywords

FED-BATCH CULTURE; HOLLOW-FIBER BIOREACTOR; RECOMBINANT ANTIBODY-PRODUCTION; FILTER PERFUSION SYSTEM; ETHANOL-PRODUCTION; ALCALIGENES-EUTROPHUS; MEMBRANE BIOREACTORS; RECYCLE BIOREACTOR; IMMOBILIZED CELLS; ESCHERICHIA-COLI

Citation

BIOTECHNOLOGY ADVANCES, v.32, no.2, pp.514 - 525

ISSN
0734-9750
DOI
10.1016/j.biotechadv.2014.01.004
URI
http://hdl.handle.net/10203/194699
Appears in Collection
CBE-Journal Papers(저널논문)
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