DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, SH | ko |
dc.contributor.author | Lee, Haeshin | ko |
dc.contributor.author | Park, TG | ko |
dc.date.accessioned | 2009-11-10T04:31:41Z | - |
dc.date.available | 2009-11-10T04:31:41Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2003-04 | - |
dc.identifier.citation | JOURNAL OF CONTROLLED RELEASE, v.89, no.2, pp.271 - 284 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.uri | http://hdl.handle.net/10203/12332 | - |
dc.description.abstract | A new class of methoxy-poly(ethylene glycol) (mPEG) derivatives having a cleavable group of oligo-lactic acid (OLA) was synthesized by ring opening polymerization Of L-lactide using a terminal hydroxyl end of mPEG as an initiator. The synthesized mPEG derivatives had 0.8 and 3.6 lactic acid units based on the MALDI-TOF data. A terminal end group of mPEG-OLA was further activated with p-nitrophenyl chloroformate to produce mPEG-OLA-p-nitrophenyl carbonate (PC). mPEG-OLA-PC derivatives were conjugated to primary amine groups of recombinant human granulocyte-colony stimulating factor. PEGylated G-CSF conjugated with mPEG-OLA-PC derivatives consisted of native, mono-, di-, and tri-PEGylated species, and they did not show any apparent conformational changes even after PEGylation. When incubating in pH 7.4 buffer at 37 degreesC for 2 days, mPEG-OLA-G-CSF conjugates liberated mPEG by cleaving the OLA spacer, resulting in the gradual regeneration of G-CSF (C) 2003 Elsevier Science B.V. All rights reserved. | - |
dc.description.sponsorship | the Ministry of Commerce, Industry and Energy (A00-961-5411-02-3-3), South Korea. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | COLONY-STIMULATING FACTOR | - |
dc.subject | POLYETHYLENE-GLYCOL | - |
dc.subject | SUPEROXIDE-DISMUTASE | - |
dc.subject | POLY(ETHYLENE GLYCOL) | - |
dc.subject | MASS-SPECTROMETRY | - |
dc.subject | IN-VIVO | - |
dc.subject | PROTEINS | - |
dc.subject | PURIFICATION | - |
dc.subject | STABILITY | - |
dc.subject | CHEMISTRY | - |
dc.title | PEGylation of G-CSF using cleavable oligo-lactic acid linkage | - |
dc.type | Article | - |
dc.identifier.wosid | 000182768400011 | - |
dc.identifier.scopusid | 2-s2.0-0037472094 | - |
dc.type.rims | ART | - |
dc.citation.volume | 89 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 271 | - |
dc.citation.endingpage | 284 | - |
dc.citation.publicationname | JOURNAL OF CONTROLLED RELEASE | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, Haeshin | - |
dc.contributor.localauthor | Park, TG | - |
dc.contributor.nonIdAuthor | Choi, SH | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | PEG | - |
dc.subject.keywordAuthor | cleavable | - |
dc.subject.keywordAuthor | G-CSF | - |
dc.subject.keywordAuthor | oligo-lactic acid | - |
dc.subject.keywordAuthor | PEGylation | - |
dc.subject.keywordPlus | COLONY-STIMULATING FACTOR | - |
dc.subject.keywordPlus | POLYETHYLENE-GLYCOL | - |
dc.subject.keywordPlus | SUPEROXIDE-DISMUTASE | - |
dc.subject.keywordPlus | POLY(ETHYLENE GLYCOL) | - |
dc.subject.keywordPlus | MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | CHEMISTRY | - |
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