A New Assay for Endonuclease/Methyltransferase Activiities Based on Graphene Oxide

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A new endonuclease/methyltransferase activity assay method based on graphene oxide (GO) is developed. Substrate DNA is designed to possess a double-stranded part to serve as a nuclease substrate and a single-stranded part for anchoring the DNA to the GO surface via strong noncovalent binding. Nuclease-mediated DNA hydrolysis induces the recovery of fluorescence intensity of the dye attached to the end of the double-stranded DNA region. This GO-based method allows real-time measurement and quantitative assay for endonuclease/methyltransferase activities in short time.
Publisher
AMER CHEMICAL SOC
Issue Date
2011
Language
English
Article Type
Article
Keywords

DNA METHYLTRANSFERASES; ENDONUCLEASE ACTIVITY; HOMOLOGOUS RECOMBINATION; S-ADENOSYLMETHIONINE; ENZYME; CANCER; REPAIR; REPLICATION; METHYLATION; INHIBITION

Citation

ANALYTICAL CHEMISTRY, v.83, no.23, pp.8906 - 8912

ISSN
0003-2700
URI
http://hdl.handle.net/10203/99922
Appears in Collection
CH-Journal Papers(저널논문)
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