Application of Polyaniline to an Enzyme-Amplified Electrochemical Immunosensor as an Electroactive Report Molecule

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Conducting polymers (CPs) are widely used as matrixes for the entrapment of enzymes in analytical chemistry and biosensing devices However, enzyme-catalyzed polymerization of CPs is rarely used for immunosensing due to the difficulties involved in the quantitative analysis of colloidal CPs in solution phase In this study, an enzyme-amplified electrocatalytic immunosensor employing a CP as a redox marker has been developed A polyanionic polymer matrix, alpha-amino-omega-thiol terminated poly(acrylic acid), was employed for precipitation of CP The acrylic acid group acts as a polyanionic template The thiol terminus of the polymer was used to produce self-assembled monolayers (SAMs) on Au electrodes and the amine terminus was employed for immobilization of biomolecules In an enzyme-amplified sandwich type immunosensor, the polyaniline (PANI) produced enzymatically is attracted by the electrostatic force of the matrix polymer The precipitated PANT was characterized by electrochemical methods
Publisher
KOREAN CHEMICAL SOC
Issue Date
2010-11
Language
English
Article Type
Article
Citation

BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.31, no.11, pp.3103 - 3108

ISSN
0253-2964
DOI
10.5012/bkcs.2010.31.11.3103
URI
http://hdl.handle.net/10203/98275
Appears in Collection
NT-Journal Papers(저널논문)CH-Journal Papers(저널논문)
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