DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hyun, Moonjung | ko |
dc.contributor.author | Park, Sojin | ko |
dc.contributor.author | Kim, Eunsun | ko |
dc.contributor.author | Kim, Do-Hyung | ko |
dc.contributor.author | Lee, Se-Jin | ko |
dc.contributor.author | Koo, Hyeon-Sook | ko |
dc.contributor.author | Seo, Yeon-Soo | ko |
dc.contributor.author | Ahn, Byungchan | ko |
dc.date.accessioned | 2013-03-11T02:20:32Z | - |
dc.date.available | 2013-03-11T02:20:32Z | - |
dc.date.created | 2012-04-18 | - |
dc.date.created | 2012-04-18 | - |
dc.date.issued | 2012-02 | - |
dc.identifier.citation | BIOCHEMISTRY, v.51, no.7, pp.1336 - 1345 | - |
dc.identifier.issn | 0006-2960 | - |
dc.identifier.uri | http://hdl.handle.net/10203/98025 | - |
dc.description.abstract | The Caenorhabditis elegans Werner syndrome protein, WRN-1, a member of the RecQ helicase family, has a 3'-5' DNA helicase activity. Worms with defective wrn-1 exhibit premature aging phenotypes and an increased level of genome instability. In response to DNA damage, WRN-1 participates in the initial stages of checkpoint activation in concert with C. elegans replication protein A (RPA-1). WRN-1 helicase is stimulated by RPA-1 on long DNA duplex substrates. However, the mechanism by which RPA-1 stimulates DNA unwinding and the function of the WRN-1-RPA-1 interaction are not clearly understood. We have found that WRN-1 physically interacts with two RPA-1 subunits, CeRPA73 and CeRPA32; however, full-length WRN-1 helicase activity is stimulated by only the CeRPA73 subunit, while the WRN-1(162-1056) fragment that harbors the helicase activity requires both the CeRPA73 and CeRPA32 subunits for the stimulation. We also found that the CeRPA73(1-464) fragment can stimulate WRN-1 helicase activity and that residues 335-464 of CeRPA73 are important for physical interaction with WRN-1. Because CeRPA73 and the CeRPA73(1-464) fragment are able to bind single-stranded DNA (ssDNA), the stimulation of WRN-1 helicase by RPA-1 is most likely due to the ssDNA binding activity of CeRPA73 and the direct interaction of WRN-1 and CeRPA73. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | WERNER-SYNDROME PROTEIN | - |
dc.subject | DOUBLE-STRAND BREAKS | - |
dc.subject | MEDIATED DNA-DAMAGE | - |
dc.subject | BLOOMS-SYNDROME PROTEIN | - |
dc.subject | SYNDROME GENE-PRODUCT | - |
dc.subject | RECQ HELICASES | - |
dc.subject | UNWINDING ACTIVITY | - |
dc.subject | REPLICATION FORKS | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | MAMMALIAN-CELLS | - |
dc.title | Physical and Functional Interactions of Caenorhabditis elegans WRN-1 Helicase with RPA-1 | - |
dc.type | Article | - |
dc.identifier.wosid | 000300473400002 | - |
dc.identifier.scopusid | 2-s2.0-84863116194 | - |
dc.type.rims | ART | - |
dc.citation.volume | 51 | - |
dc.citation.issue | 7 | - |
dc.citation.beginningpage | 1336 | - |
dc.citation.endingpage | 1345 | - |
dc.citation.publicationname | BIOCHEMISTRY | - |
dc.identifier.doi | 10.1021/bi200791p | - |
dc.contributor.localauthor | Seo, Yeon-Soo | - |
dc.contributor.nonIdAuthor | Hyun, Moonjung | - |
dc.contributor.nonIdAuthor | Park, Sojin | - |
dc.contributor.nonIdAuthor | Kim, Eunsun | - |
dc.contributor.nonIdAuthor | Kim, Do-Hyung | - |
dc.contributor.nonIdAuthor | Lee, Se-Jin | - |
dc.contributor.nonIdAuthor | Koo, Hyeon-Sook | - |
dc.contributor.nonIdAuthor | Ahn, Byungchan | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | WERNER-SYNDROME PROTEIN | - |
dc.subject.keywordPlus | DOUBLE-STRAND BREAKS | - |
dc.subject.keywordPlus | MEDIATED DNA-DAMAGE | - |
dc.subject.keywordPlus | BLOOMS-SYNDROME PROTEIN | - |
dc.subject.keywordPlus | SYNDROME GENE-PRODUCT | - |
dc.subject.keywordPlus | RECQ HELICASES | - |
dc.subject.keywordPlus | UNWINDING ACTIVITY | - |
dc.subject.keywordPlus | REPLICATION FORKS | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
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