Time variation of fluorescence lifetime in enhanced cyan fluorescence protein

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The lifetime variations of enhanced cyan fluorescence protein (ECFP) in relatively short integration time bins were studied via time-correlated single photon counting (TCSPC) measurement. We observed that minimum photon counts are necessary for the lifetime estimation to achieve a certain range of variance. The conditions to decrease the variance of lifetime were investigated and the channel width of the measurement of TCSPC data was found to be another important factor for the variance of lifetime. Though the lifetime of ECFP is best fit by a double exponential, a mono exponential fit for the same integration time is more stable. The results may be useful in the analysis of photophysical dynamics for ensemble molecules in short measurement time windows. (C) 2010 Elsevier B.V. All rights reserved.
Publisher
ELSEVIER SCIENCE BV
Issue Date
2010-07
Language
English
Article Type
Article
Keywords

ENERGY-TRANSFER; MOLECULES; DYNAMICS; BLINKING; VARIANT; FRET; CELL

Citation

JOURNAL OF LUMINESCENCE, v.130, pp.1300 - 1304

ISSN
0022-2313
DOI
10.1016/j.jlumin.2010.02.043
URI
http://hdl.handle.net/10203/96790
Appears in Collection
PH-Journal Papers(저널논문)
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