Protein micropatterning on bifunctional organic-inorganic sol-gel hybrid materials

Cited 27 time in webofscience Cited 0 time in scopus
  • Hit : 481
  • Download : 134
Active protein micropatterns and microarrays made by selective localization are popular candidates for medical diagnostics, such as biosensors, bioMEMS, and basic protein studies. In this paper, we present a simple fabrication process of thick (similar to 20 mu m) protein micropatterning using capillary force lithography with bifunctional sol-gel hybrid materials. Because bifunctional sol-gel hybrid material can have both an amine function for linking with protein and a methacryl function for photocuring, proteins such as streptavidin can be immobilized directly on thick bifunctional sol-gel hybrid micropatterns. Another advantage of the bifunctional sol-gel hybrid materials is the high selective stability of the amine group on bifunctional sol-gel hybrid patterns. Because amine function is regularly contained in each siloxane oligomers, immobilizing sites for streptavidin are widely distributed on the surface of thick hybrid micropatterns. The micropatterning processes of active proteins using efficient bifunctional sol-gel hybrid materials will be useful for the development of future bioengineered systems because they can save several processing steps and reduce costs.
Publisher
Amer Chemical Soc
Issue Date
2007-04
Language
English
Article Type
Article
Keywords

DIP-PEN NANOLITHOGRAPHY; PATTERNS; FABRICATION; PHOTOPOLYMERIZATION; MICROSTRUCTURES; LITHOGRAPHY; TECHNOLOGY; SURFACES; CELLS

Citation

LANGMUIR, v.23, no.9, pp.4732 - 4736

Citation
Langmuir, Vol. 23, No.9, pp.4732-4736
ISSN
0743-7463
URI
http://hdl.handle.net/10203/9679
Appears in Collection
MS-Journal Papers(저널논문)
Files in This Item
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 27 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0