Improvement of the axial resolution in confocal microscopy by the use of heterodyne interference

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dc.contributor.authorLee, Seungwooko
dc.contributor.authorGweon, Dae-Gabko
dc.date.accessioned2013-03-08T05:18:50Z-
dc.date.available2013-03-08T05:18:50Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2008-10-
dc.identifier.citationMEASUREMENT SCIENCE & TECHNOLOGY, v.19, no.10-
dc.identifier.issn0957-0233-
dc.identifier.urihttp://hdl.handle.net/10203/92227-
dc.description.abstractA technique for improving the axial resolution of confocal microscopy is proposed and demonstrated. Based on the interference between two diffraction-limited spots separated along the optical axis, a field domain confined by the heterodyne interference is generated. The effective region of illumination made by the interference makes the point-spread function (PSF) of the confocal microscope sharper. The three-dimensional imaging equations for both coherent and incoherent systems are derived. Numerical simulation and experiment show that the full-width-at-half-maximum (FWHM) of the PSF can be improved by a factor of 1.78 and 1.41, respectively.-
dc.languageEnglish-
dc.publisherIOP PUBLISHING LTD-
dc.subject3D WIDEFIELD MICROSCOPY-
dc.subjectPOINT-SPREAD FUNCTION-
dc.subject2 OBJECTIVE LENSES-
dc.subjectFLUORESCENCE MICROSCOPY-
dc.subject2-PHOTON EXCITATION-
dc.subjectSCANNING MICROSCOPE-
dc.subjectLIGHT-MICROSCOPY-
dc.subjectSUPERRESOLUTION-
dc.subjectRESTORATION-
dc.subjectFILTERS-
dc.titleImprovement of the axial resolution in confocal microscopy by the use of heterodyne interference-
dc.typeArticle-
dc.identifier.wosid000259113400018-
dc.identifier.scopusid2-s2.0-58149314320-
dc.type.rimsART-
dc.citation.volume19-
dc.citation.issue10-
dc.citation.publicationnameMEASUREMENT SCIENCE & TECHNOLOGY-
dc.identifier.doi10.1088/0957-0233/19/10/105502-
dc.contributor.localauthorGweon, Dae-Gab-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorconfocal microscopy-
dc.subject.keywordAuthorheterodyne interference-
dc.subject.keywordAuthoraxial resolution-
dc.subject.keywordAuthordeconvolution-
dc.subject.keywordPlus3D WIDEFIELD MICROSCOPY-
dc.subject.keywordPlusPOINT-SPREAD FUNCTION-
dc.subject.keywordPlus2 OBJECTIVE LENSES-
dc.subject.keywordPlusFLUORESCENCE MICROSCOPY-
dc.subject.keywordPlus2-PHOTON EXCITATION-
dc.subject.keywordPlusSCANNING MICROSCOPE-
dc.subject.keywordPlusLIGHT-MICROSCOPY-
dc.subject.keywordPlusSUPERRESOLUTION-
dc.subject.keywordPlusRESTORATION-
dc.subject.keywordPlusFILTERS-
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