DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bochkareva, E | ko |
dc.contributor.author | Kaustov, L | ko |
dc.contributor.author | Ayed, A | ko |
dc.contributor.author | Yi, Gwan-Su | ko |
dc.contributor.author | Lu, Y | ko |
dc.contributor.author | Pineda-Lucena, A | ko |
dc.contributor.author | Liao, JCC | ko |
dc.contributor.author | Okorokov, AL | ko |
dc.contributor.author | Milner, J | ko |
dc.contributor.author | Arrowsmith, CH | ko |
dc.contributor.author | Bochkarev, A | ko |
dc.date.accessioned | 2013-03-07T12:25:08Z | - |
dc.date.available | 2013-03-07T12:25:08Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2005-10 | - |
dc.identifier.citation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.102, no.43, pp.15412 - 15417 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/10203/90177 | - |
dc.description.abstract | one of many protein-protein interactions modulated upon DNA damage is that of the single-stranded DNA-binding protein, replication protein A (RPA), with the p53 tumor suppressor. Here we report the crystal structure of RPA residues 1-120 (RPA70N) bound to the N-terminal transactivation domain of p53 (residues 37-57; p53N) and, by using NMR spectroscopy, characterize two mechanisms by which the RPA/p53 interaction can be modulated. RPA70N forms an oligonucleotide/oligosaccharide-binding fold, similar to that previously observed for the ssDNA-binding domains of RPA. In contrast, the N-terminal p53 transactivation domain is largely disordered in solution, but residues 37-57 fold into two amphipathic helices, H1 and H2, upon binding with RPA70N. The H2 helix of p53 structurally mimics the binding of ssDNA to the oligonucleotide/oligosaccharide-binding fold. NMR experiments confirmed that both ssDNA and an acidic peptide mimicking a phosphorylated form of RPA32N can independently compete the acidic p53N out of the binding site. Taken together, our data suggest a mechanism for DNA damage signaling that can explain a threshold response to DNA damage. | - |
dc.language | English | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.subject | INDUCED PHOSPHORYLATION | - |
dc.subject | ATAXIA-TELANGIECTASIA | - |
dc.subject | BINDING-DOMAIN | - |
dc.subject | UV-RADIATION | - |
dc.subject | KDA SUBUNIT | - |
dc.subject | RPA | - |
dc.subject | DAMAGE | - |
dc.subject | COMPLEX | - |
dc.subject | KINASE | - |
dc.subject | BRCA2 | - |
dc.title | Single-stranded DNA mimicry in the p53 transactivation domain interaction with replication protein A | - |
dc.type | Article | - |
dc.identifier.wosid | 000232929400028 | - |
dc.identifier.scopusid | 2-s2.0-27344449049 | - |
dc.type.rims | ART | - |
dc.citation.volume | 102 | - |
dc.citation.issue | 43 | - |
dc.citation.beginningpage | 15412 | - |
dc.citation.endingpage | 15417 | - |
dc.citation.publicationname | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.identifier.doi | 10.1073/pnas.0504614102 | - |
dc.contributor.localauthor | Yi, Gwan-Su | - |
dc.contributor.nonIdAuthor | Bochkareva, E | - |
dc.contributor.nonIdAuthor | Kaustov, L | - |
dc.contributor.nonIdAuthor | Ayed, A | - |
dc.contributor.nonIdAuthor | Lu, Y | - |
dc.contributor.nonIdAuthor | Pineda-Lucena, A | - |
dc.contributor.nonIdAuthor | Liao, JCC | - |
dc.contributor.nonIdAuthor | Okorokov, AL | - |
dc.contributor.nonIdAuthor | Milner, J | - |
dc.contributor.nonIdAuthor | Arrowsmith, CH | - |
dc.contributor.nonIdAuthor | Bochkarev, A | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | DNA binding | - |
dc.subject.keywordAuthor | protein-protein interaction | - |
dc.subject.keywordAuthor | structural analysis | - |
dc.subject.keywordAuthor | ssDNA mimicry | - |
dc.subject.keywordPlus | INDUCED PHOSPHORYLATION | - |
dc.subject.keywordPlus | ATAXIA-TELANGIECTASIA | - |
dc.subject.keywordPlus | BINDING-DOMAIN | - |
dc.subject.keywordPlus | UV-RADIATION | - |
dc.subject.keywordPlus | KDA SUBUNIT | - |
dc.subject.keywordPlus | RPA | - |
dc.subject.keywordPlus | DAMAGE | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | BRCA2 | - |
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