Molecular tripods showing fluorescence enhancement upon binding to streptavidin

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We introduce a new approach to biotin-streptavidin assays based on a molecular tripod which consists of biotin, a fluorophore, and a quencher. The interaction between streptavidin and molecular tripods perturbs the ground-state quencher-fluorophore dimeric conformation in the absence of streptavidin and diminishes the intrinsic self-quenching of a quencher-fluorophore pair. The emission intensity of the molecular tripods plus streptavidin is 3.5-5.2 times that of molecular tripods in the absence of streptavidin.
Publisher
AMER CHEMICAL SOC
Issue Date
2005-01
Language
English
Article Type
Article
Keywords

POLYPYRIDINE BIOTIN COMPLEXES; POLY(ETHYLENE GLYCOL) SPACERS; INTRAMOLECULAR DIMERS; LUMINESCENCE ENHANCEMENT; AVIDIN; PROBES; CYCLODEXTRIN; CONJUGATE; BIOFLUIDS; STRATEGY

Citation

ORGANIC LETTERS, v.7, no.1, pp.111 - 114

ISSN
1523-7060
DOI
10.1021/ol047801h
URI
http://hdl.handle.net/10203/87876
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