DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ahmad, Raza | ko |
dc.contributor.author | Kim, Yun-Hee | ko |
dc.contributor.author | Kim, Myoung Duck | ko |
dc.contributor.author | Phung, Minh-Ngoc | ko |
dc.contributor.author | Chung, Won Il | ko |
dc.contributor.author | Lee, Haeng-Soon | ko |
dc.contributor.author | Kwak, Sang-Soo | ko |
dc.contributor.author | Kwon, Sulk-Yoon | ko |
dc.date.accessioned | 2013-03-06T17:48:02Z | - |
dc.date.available | 2013-03-06T17:48:02Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2008-11 | - |
dc.identifier.citation | JOURNAL OF PLANT BIOLOGY, v.51, no.6, pp.401 - 407 | - |
dc.identifier.issn | 1226-9239 | - |
dc.identifier.uri | http://hdl.handle.net/10203/87838 | - |
dc.description.abstract | A binary vector devoid of a plant selection-marker gene (designated as pSSA-F) was constructed to overcome bio-safety concerns about genetically modified plants. This vector carried chloroplast-targeted superoxide dismutase (SOD) and ascorbate peroxidase (APX) genes under the control of an oxidative stress-inducible (SWPA2) promoter, and was utilized to transform potato (Solanum tuberosum L.). integration of these foreign genes into transgenic plants was primarily performed via PCR with genomic DNA. Twelve marker-free transgenic lines were obtained by inoculating stem explants. The maximum transformation efficiency was 6.25% and averaged 2.2%. Successful integration of the SOD and APX genes rendered transgenic plants tolerant to methyl viologen-mediated oxidative stress at the leaf-disc and whole-plant levels. Our findings suggest that this technique for developing selection marker-free transgenic plants is feasible and can be employed with other crop species. | - |
dc.language | English | - |
dc.publisher | Springer | - |
dc.subject | SUPEROXIDE-DISMUTASE | - |
dc.subject | SWEET-POTATO | - |
dc.subject | GENE | - |
dc.subject | CHLOROPLASTS | - |
dc.subject | TRANSFORMATION | - |
dc.subject | PEROXIDASE | - |
dc.subject | EXPRESSION | - |
dc.subject | GROWTH | - |
dc.subject | SALT | - |
dc.subject | BIOSAFETY | - |
dc.title | Development of Selection Marker-free Transgenic Potato Plants with Enhanced Tolerance to Oxidative Stress | - |
dc.type | Article | - |
dc.identifier.wosid | 000261747300003 | - |
dc.identifier.scopusid | 2-s2.0-63849230810 | - |
dc.type.rims | ART | - |
dc.citation.volume | 51 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 401 | - |
dc.citation.endingpage | 407 | - |
dc.citation.publicationname | JOURNAL OF PLANT BIOLOGY | - |
dc.identifier.doi | 10.1007/BF03036060 | - |
dc.contributor.localauthor | Chung, Won Il | - |
dc.contributor.nonIdAuthor | Ahmad, Raza | - |
dc.contributor.nonIdAuthor | Kim, Yun-Hee | - |
dc.contributor.nonIdAuthor | Kim, Myoung Duck | - |
dc.contributor.nonIdAuthor | Phung, Minh-Ngoc | - |
dc.contributor.nonIdAuthor | Lee, Haeng-Soon | - |
dc.contributor.nonIdAuthor | Kwak, Sang-Soo | - |
dc.contributor.nonIdAuthor | Kwon, Sulk-Yoon | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | genetic transformation | - |
dc.subject.keywordAuthor | marker-free transgenic plants | - |
dc.subject.keywordAuthor | oxidative stress | - |
dc.subject.keywordAuthor | potato | - |
dc.subject.keywordPlus | SUPEROXIDE-DISMUTASE | - |
dc.subject.keywordPlus | SWEET-POTATO | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | CHLOROPLASTS | - |
dc.subject.keywordPlus | TRANSFORMATION | - |
dc.subject.keywordPlus | PEROXIDASE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | SALT | - |
dc.subject.keywordPlus | BIOSAFETY | - |
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