DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최요한 | ko |
dc.contributor.author | 손상욱 | ko |
dc.contributor.author | 이승섭 | ko |
dc.date.accessioned | 2013-03-05T00:36:26Z | - |
dc.date.available | 2013-03-05T00:36:26Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2004-08 | - |
dc.identifier.citation | 대한기계학회논문집 A, v.28, no.8, pp.1183 - 1189 | - |
dc.identifier.issn | 1226-4873 | - |
dc.identifier.uri | http://hdl.handle.net/10203/84907 | - |
dc.description.abstract | A novel cell count method, which can improve the count efficiency and reduce contamination problem, was presented using micro lattice engraved on culture dish. The micro lattice has feature of $50{\mu}m{\times}50{\mu}m$ rectangular shape, 2㎛ line width, and 2㎛ depth in $3mm{\times}3mm$ area. In this paper, nickel mold was fabricated with thickness of 3mm and diameter of 80 mm, and transcription of the micro lattice on a polystyrene cell culture dish was performed by hot embossing at $200\;^{\circ}C$. The tedious and error-prone harvest/load processes of conventional cell counts with a hemocytometer could be omitted, and these advantages became magnified during periodical counts involving long-term cultures. SupT1 cells and HeLa cells were cultivated with the dish for 7 days in $CO2 incubator and counted as $371.84/mm^2$ and $123.36/mm^2$, respectively, during the cultivation without harmful effects on the cells. | - |
dc.language | Korean | - |
dc.publisher | 대한기계학회 | - |
dc.title | 미세 격자가 형성된 배양 접시를 이용한 새로운 세포 계수 방법 | - |
dc.title.alternative | A Novel Cell Count Method Using Micro Lattice Engraved On A Culture Dish | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.citation.volume | 28 | - |
dc.citation.issue | 8 | - |
dc.citation.beginningpage | 1183 | - |
dc.citation.endingpage | 1189 | - |
dc.citation.publicationname | 대한기계학회논문집 A | - |
dc.identifier.kciid | ART000974688 | - |
dc.contributor.localauthor | 이승섭 | - |
dc.contributor.nonIdAuthor | 최요한 | - |
dc.contributor.nonIdAuthor | 손상욱 | - |
dc.subject.keywordAuthor | Cell count | - |
dc.subject.keywordAuthor | Culture dish | - |
dc.subject.keywordAuthor | Micro lattice | - |
dc.subject.keywordAuthor | Hemocytomter | - |
dc.subject.keywordAuthor | Hot embossing | - |
dc.subject.keywordAuthor | Cell count | - |
dc.subject.keywordAuthor | Culture dish | - |
dc.subject.keywordAuthor | Micro lattice | - |
dc.subject.keywordAuthor | Hemocytomter | - |
dc.subject.keywordAuthor | Hot embossing | - |
dc.subject.keywordAuthor | 세포 계수 | - |
dc.subject.keywordAuthor | 배양 접시 | - |
dc.subject.keywordAuthor | 미세 격자 | - |
dc.subject.keywordAuthor | 헤모사이토미터 | - |
dc.subject.keywordAuthor | 핫 엠보싱 | - |
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