Dna2 requirement for normal reproduction of Caenorhabditis elegans is temperature-dependent

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To determine the function of Dna2 in a multicellular organism, the Caenorhabditis elegans Dna2 expression was probed and deletion mutant phenotypes were analyzed. Dna2 was localized to the nuclei of C. elegans oocytes and early embryos by immunostaining or green fluorescent protein-tagging. A homozygous dna2 deletion mutant showed a reduced brood size and embryonic lethality, and the phenotypes greatly depended on growth temperature and aggravated in the succeeding generation. The mutant embryos also showed delayed cell divisions, which together with temperature-dependence of the mutant phenotypes supported the well-conserved role of Dna2 in DNA replication.
Publisher
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
Issue Date
2003-02
Language
English
Article Type
Article
Keywords

SINGLE-STRANDED-DNA; ESSENTIAL IN-VIVO; ENDONUCLEASE ACTIVITY; GERMLINE DEVELOPMENT; C-ELEGANS; REPLICATION; HELICASE; PROTEIN; ENCODES; MUTATIONS

Citation

MOLECULES AND CELLS, v.15, pp.81 - 86

ISSN
1016-8478
URI
http://hdl.handle.net/10203/81327
Appears in Collection
BS-Journal Papers(저널논문)
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