DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, JS | ko |
dc.contributor.author | Shin, Eui-Cheol | ko |
dc.contributor.author | Kim, JS | ko |
dc.contributor.author | Choi, IH | ko |
dc.contributor.author | Park, JH | ko |
dc.contributor.author | Kim, SJ | ko |
dc.date.accessioned | 2013-03-03T07:29:49Z | - |
dc.date.available | 2013-03-03T07:29:49Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1999-12 | - |
dc.identifier.citation | HYBRIDOMA, v.18, no.6, pp.521 - 527 | - |
dc.identifier.issn | 0272-457X | - |
dc.identifier.uri | http://hdl.handle.net/10203/77776 | - |
dc.description.abstract | Human natural killer (NK) cells have receptors that bind to HLA class I molecules. Among these receptors, p58 and p50 bind to HLA-C, P58 belongs to the killer inhibitory receptor (KIR) and p50 belongs to the killer activatory receptor (KAR), Previously, we obtained three recombinant p58 KIR or p50 KAR proteins, KAR-K1 (KIR2DS4), KIR-K6 (KIR2DL1), and KIR-K7 (KIR2DL3). In this study, we produced and characterized seven monoclonal antibodies (MAbs) to the p58 KIR and p50 KAR proteins. The MAbs were classified in three groups according to their antigen-binding specificity: 5IB103 and 26ID707 were KAR-K1-specific; A809, 190IIC311, and 197IIC611 were KIR-K7-specific; while A210 and A803g bound to all three recombinant proteins. The MAbs reactive to KIR-K7 bound to the gamma 3 domain among two immunoglobulin (Ig) domains of KIR-K7, Immunofluorescence staining and flow cytometric analysis with A803g showed reactivity to about 10% of peripheral blood mononuclear cells and 35% of purified natural killer cells. Double immunofluorescence staining with A803g and anti-CD56 Ab showed that CD56 and p58 or p50 were expressed on NK cells in a mutually exclusive way, We also investigated T-cell markers in A803g(+) cells. A803g(+) T cells were almost CD8(+) cells. MAbs produced in this study can be utilized practically in the investigation of biological characteristics of p58 KIR and p50 KAR. | - |
dc.language | English | - |
dc.publisher | MARY ANN LIEBERT INC PUBL | - |
dc.subject | MAJOR HISTOCOMPATIBILITY COMPLEX | - |
dc.subject | CLASS-I MOLECULES | - |
dc.subject | CELL RECEPTORS | - |
dc.subject | NK CELLS | - |
dc.subject | HLA-C | - |
dc.subject | RECOGNITION | - |
dc.subject | ACTIVATION | - |
dc.subject | SUBSET | - |
dc.subject | IDENTIFICATION | - |
dc.subject | EXPRESSION | - |
dc.title | Monoclonal antibodies with various reactivity to p58 killer inhibitory receptors | - |
dc.type | Article | - |
dc.identifier.wosid | 000084499200007 | - |
dc.identifier.scopusid | 2-s2.0-0033394390 | - |
dc.type.rims | ART | - |
dc.citation.volume | 18 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 521 | - |
dc.citation.endingpage | 527 | - |
dc.citation.publicationname | HYBRIDOMA | - |
dc.contributor.localauthor | Shin, Eui-Cheol | - |
dc.contributor.nonIdAuthor | Shin, JS | - |
dc.contributor.nonIdAuthor | Kim, JS | - |
dc.contributor.nonIdAuthor | Choi, IH | - |
dc.contributor.nonIdAuthor | Park, JH | - |
dc.contributor.nonIdAuthor | Kim, SJ | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | MAJOR HISTOCOMPATIBILITY COMPLEX | - |
dc.subject.keywordPlus | CLASS-I MOLECULES | - |
dc.subject.keywordPlus | CELL RECEPTORS | - |
dc.subject.keywordPlus | NK CELLS | - |
dc.subject.keywordPlus | HLA-C | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | SUBSET | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
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