DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, D.C. | ko |
dc.contributor.author | Lee, S.G. | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2013-03-03T06:07:27Z | - |
dc.date.available | 2013-03-03T06:07:27Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1996 | - |
dc.identifier.citation | ENZYME AND MICROBIAL TECHNOLOGY, v.18, no.1, pp.35 - 40 | - |
dc.identifier.issn | 0141-0229 | - |
dc.identifier.uri | http://hdl.handle.net/10203/77565 | - |
dc.description.abstract | Thermostable D-hydantoinase from thermophilic Bacillus stearothermophilus SD-1 was used to produce N-carbamoyl-D-p-hydroxyphenylglycine (NC-HPG) from D, L-5-(4-hydroxyphenyl)/hydantoin (DL-5-HPH). Culture conditions for the production of the enzyme from B. stearothermophilus SD-1 were optimized. The D-hydantoinase was immobilized on various support matrices by adsorption, and DEAE-cellulose resin was Sound to be most effective ill terms of the activity recovery and the amount of protein bound. The activity of enzyme immobilized on DEAE-cellulose was retained >90%, and the optimal reaction conditions for the immobilized enzyme were determined to be 55 degrees C and pH 9.0, respectively. Immobilized enzyme was applied to the production of NC-HPG from DL-5-HPH in repealed hatch, and the production rate was maintained constantly over nine successive operations. | - |
dc.language | English | - |
dc.publisher | BUTTERWORTH-HEINEMANN | - |
dc.subject | MICROBIAL TRANSFORMATION | - |
dc.subject | AMINO-ACIDS | - |
dc.title | Production of D-p-hydroxyphenylglycine from D,L-5-(4-hydroxyphenyl)hydantoin using immobilized thermostable D-hydantoinase from Bacillus stearothermophilus SD-1 | - |
dc.type | Article | - |
dc.identifier.wosid | A1996TT61400007 | - |
dc.type.rims | ART | - |
dc.citation.volume | 18 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 35 | - |
dc.citation.endingpage | 40 | - |
dc.citation.publicationname | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.identifier.doi | 10.1016/0141-0229(96)00062-2 | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.nonIdAuthor | Lee, D.C. | - |
dc.contributor.nonIdAuthor | Lee, S.G. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Thermostable D-hydantoinase | - |
dc.subject.keywordAuthor | Bacillus stearothermophilus | - |
dc.subject.keywordAuthor | immobilization | - |
dc.subject.keywordAuthor | D-p-hydroxyphenylglycine | - |
dc.subject.keywordAuthor | D,L-5-(4-hydroxyphenyl)hydantoin | - |
dc.subject.keywordPlus | MICROBIAL TRANSFORMATION | - |
dc.subject.keywordPlus | AMINO-ACIDS | - |
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